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PXD035227

PXD035227 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHuman HEK293 Gemin5 phosphorylation site T897 mutants LC-MSMS
DescriptionIdentification of cellular proteins interacting with Gemin5 phosphorylation mutants in T897 residue. We used the T897A mutant as phosphorylation defective protein and the T897E mutant as phosphomimetic. P85 C-terminal fragment of Gemin5 has been used to purify the protein complexes associated by Tandem Affinity Purification. The study has been performed using two biological replicates of each bait protein: p85-T897A and p85-T897E.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:37:57.217.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD035227
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterRosario Francisco-Velilla
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-07-11 08:04:51ID requested
12023-03-10 23:07:59announced
22023-11-14 08:31:52announced2023-11-14: Updated project metadata.
32023-11-14 08:37:58announced2023-11-14: Updated project metadata.
Publication List
10.6019/PXD035227;
Francisco-Velilla R, Embarc-Buh A, Del Ca, ñ, o-Ochoa F, Abellan S, Vilar M, Alvarez S, Fernandez-Jaen A, Kour S, Rajan DS, Pandey UB, Ram, ó, n-Maiques S, Martinez-Salas E, Functional and structural deficiencies of Gemin5 variants associated with neurological disorders. Life Sci Alliance, 5(7):(2022) [pubmed]
Francisco-Velilla R, Embarc-Buh A, Abellan S, Del Ca, ñ, o-Ochoa F, Ram, ó, n-Maiques S, Martinez-Salas E, Phosphorylation of T897 in the dimerization domain of Gemin5 modulates protein interactions and translation regulation. Comput Struct Biotechnol J, 20():6182-6191(2022) [pubmed]
Keyword List
submitter keyword: Human, Gemin5, HEK293, Phosphorylation
Contact List
Encarnacion Martinez-Salas
contact affiliationGenome Dynamics and Function Program Centro de Biologia Molecular Severo Ochoa Madrid, Spain
contact emailemartinez@cbm.csic.es
lab head
Rosario Francisco-Velilla
contact affiliationCBMSO
contact emailrfrancisco@cbm.csic.es
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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