PXD035227 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Human HEK293 Gemin5 phosphorylation site T897 mutants LC-MSMS |
Description | Identification of cellular proteins interacting with Gemin5 phosphorylation mutants in T897 residue. We used the T897A mutant as phosphorylation defective protein and the T897E mutant as phosphomimetic. P85 C-terminal fragment of Gemin5 has been used to purify the protein complexes associated by Tandem Affinity Purification. The study has been performed using two biological replicates of each bait protein: p85-T897A and p85-T897E. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:37:57.217.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD035227 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Rosario Francisco-Velilla |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-07-11 08:04:51 | ID requested | |
1 | 2023-03-10 23:07:59 | announced | |
2 | 2023-11-14 08:31:52 | announced | 2023-11-14: Updated project metadata. |
⏵ 3 | 2023-11-14 08:37:58 | announced | 2023-11-14: Updated project metadata. |
Publication List
10.6019/PXD035227; |
Francisco-Velilla R, Embarc-Buh A, Del Ca, ñ, o-Ochoa F, Abellan S, Vilar M, Alvarez S, Fernandez-Jaen A, Kour S, Rajan DS, Pandey UB, Ram, ó, n-Maiques S, Martinez-Salas E, Functional and structural deficiencies of Gemin5 variants associated with neurological disorders. Life Sci Alliance, 5(7):(2022) [pubmed] |
Francisco-Velilla R, Embarc-Buh A, Abellan S, Del Ca, ñ, o-Ochoa F, Ram, ó, n-Maiques S, Martinez-Salas E, Phosphorylation of T897 in the dimerization domain of Gemin5 modulates protein interactions and translation regulation. Comput Struct Biotechnol J, 20():6182-6191(2022) [pubmed] |
Keyword List
submitter keyword: Human, Gemin5, HEK293, Phosphorylation |
Contact List
Encarnacion Martinez-Salas |
contact affiliation | Genome Dynamics and Function Program Centro de Biologia Molecular Severo Ochoa Madrid, Spain |
contact email | emartinez@cbm.csic.es |
lab head | |
Rosario Francisco-Velilla |
contact affiliation | CBMSO |
contact email | rfrancisco@cbm.csic.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035227
- Label: PRIDE project
- Name: Human HEK293 Gemin5 phosphorylation site T897 mutants LC-MSMS