PXD035212

PXD035212 is an original dataset announced via ProteomeXchange.

Title	Identification of PP1c-PPP1R12A Substrates Using K-BIPS (Kinase-Catalyzed Biotinylation to Identify Phosphatase Substrates)
Description	Protein phosphatase 1 regulatory subunit 12A (PPP1R12A) interacts with the catalytic subunit of protein phosphatase 1 (PP1c) to form one of the many protein phosphatase 1 complexes, PP1c-PPP1R12A (or myosin phosphatase). In addition to a well-documented role in muscle contraction, the PP1c-PPP1R12A complex is associated with cytoskeleton organization, cell migration, adhesion, cell division, and insulin signaling. Despite the variety of biological functions, only a few substrates of the PP1c-PPP1R12A complex are characterized, which limits a full understanding of PP1c-PPP1R12A activities. Here, the chemoproteomics method K-BIPS (Kinase-catalyzed Biotinylation to Identify Phosphatase Substrates) was used to identify substrates of the PP1c-PPP1R12A complex in L6 skeletal muscle cells. K-BIPS enriched 136 candidate substrates with 14 high confidence hits. Two high confidence hits, AKT1 kinase and COPS4 signalosome proteins, were studied as novel PP1c-PPP1R12A substrates. Interestingly, both AKT1 and COPS4 were previously documented to regulate PP1c-PPP1R12A phosphatase activity. Therefore, the data suggest that PP1c-PPP1R12A influences its own phosphatase activity through substrate-dependent feedback mechanisms
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:06:41.842.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD035212
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Eric Davis
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-11 02:39:05	ID requested
1	2023-10-24 10:51:25	announced
⏵ 2	2023-11-14 09:06:50	announced	2023-11-14: Updated project metadata.

Dedigama-Arachchige PM, Acharige NPN, Zhang X, Bremer HJ, Yi Z, Pflum MKH, Identification of PP1c-PPP1R12A Substrates Using Kinase-Catalyzed Biotinylation to Identify Phosphatase Substrates. ACS Omega, 8(39):35628-35637(2023) [pubmed]

10.6019/PXD035212;

submitter keyword: Protein Phosphatase 1

PP1

PPP1R12A

chemoproteomics

substrate identification

Mary K Pflum
contact affiliation	Wayne State University Chemistry
contact email	pflum@wayne.edu
lab head
Eric Davis
contact affiliation	Wayne State University
contact email	gm9453@wayne.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD035212

PRIDE project URI

• PRIDE
  1. PXD035212
     1. Label: PRIDE project
     2. Name: Identification of PP1c-PPP1R12A Substrates Using K-BIPS (Kinase-Catalyzed Biotinylation to Identify Phosphatase Substrates)