PXD035194

PXD035194 is an original dataset announced via ProteomeXchange.

Title	Monoclonal antibody affinity-enrichment pull-down proteomics for Fasciola hepatica gut-associated proteins
Description	The zoonotic liver fluke Fasciola hepatica is responsible for economic losses to the agriculture industry worldwide. Triclabendazole has been the drug of choice. However, anthelmintic resistance is becoming prevalent with cases of resistance detected globally. This emphasizes the need for alternative control methods. A significant focus is on the development of protein vaccines. In this process however, glycoproteins (GPs) have been mostly ignored. GPs are present throughout the organ systems of F. hepatica and such gut-associated GPs could serve as ‘hidden’ antigens. Hidden antigens are not initially exposed to the host’s immune system during the course of natural infections, but have proven to be effective vaccine candidates, notably for antigens that are present in the intestine of blood-feeding and tissue-dwelling parasites. This work describes the purification of gut-associated parasite GPs based on protective antibodies against adult flukes in vitro, which demonstrate a binding on fluke gut structures. Finally, we provide a list of promising novel F. hepatica gut-associated vaccine candidates.
HostingRepository	PRIDE
AnnounceDate	2026-06-29
AnnouncementXML	Submission_2026-06-28_16:47:55.468.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD035194
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ramon Eichenberger
SpeciesList	scientific name: Fasciola hepatica (Liver fluke); NCBI TaxID: NEWT:6192;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-09 08:51:10	ID requested
⏵ 1	2026-06-28 16:47:56	announced

Rechsteiner SG, Hasler S, Gehrig P, Deplazes P, Eichenberger RM, gut-associated glycoproteins as potential vaccine candidates by lectin-affinity chromatography, flukicidal monoclonal antibodies, and affinity-enrichment mass spectrometry. Front Parasitol, 5():1746419(2026) [pubmed]

10.3389/fpara.2026.1746419;

10.6019/PXD035194;

submitter keyword: Fasciola hepatica, monoclonal antibody, gut antigen, proteomics, mass spectrometry, pull-down, liver fluke

Ramon Marc Eichenberger
contact affiliation	Institute of Parasitology, University of Zurich, Zurich, Switzerland
contact email	ramon.eichenberger@uzh.ch
lab head
Ramon Eichenberger
contact affiliation	Institute of Parasitology, University of Zurich, Zurich, Switzerland
contact email	ramon.eichenberger@uzh.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD035194

PRIDE project URI

• PRIDE
  1. PXD035194
     1. Label: PRIDE project
     2. Name: Monoclonal antibody affinity-enrichment pull-down proteomics for Fasciola hepatica gut-associated proteins