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PXD035192

PXD035192 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleYeast Mitochondrial Derived Vesicles (MDVs) have membrane potential and contain a functional ATP synthase
DescriptionMitochondria are responsible for the production of energy and for essential metabolic pathways in the cell, as well as fundamental processes such as apoptosis and aging. Interestingly, while the import machinery of proteins into mitochondria is thoroughly understood, there is currently no known protein export mechanism from mitochondria. Vesicular transport is a means of inter-organellar communication, and in fact cells release vesicles in order to shuttle lipids, proteins, RNA and DNA between one another. Here we show for the first time that functional mitochondria, isolated from Saccharomyces cerevisiae can release vesicles, independent of the fission machinery. Mitochondrial secreted vesicles are relatively uniform in size, of about 100nm and carry a selection of cargo; only certain proteins are carried by these vesicles, of which some are matrix soluble and some are membrane proteins. We further demonstrate that yeast MDVs harbor a membrane potential, a functional ATP synthase complex, produce ATP, and fuse with naïve mitochondria. We suggest, that MDVs may have a function in organellar protein export, ATP production ability of damaged mitochondria and organelle-communication.
HostingRepositoryPRIDE
AnnounceDate2023-01-29
AnnouncementXMLSubmission_2023-01-29_00:05:43.891.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTamarZiv
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-07-09 08:49:02ID requested
12023-01-29 00:05:44announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Mitochondrial Derived Vesicles, ATP synthase
Contact List
PinesOphry
contact affiliationDepartment of Molecular Genetics and Microbiology, IMRIC, Faculty of Medicine, Hebrew University, Jerusalem, Israel
contact emailophryp@ekmd.huji.ac.il
lab head
TamarZiv
contact affiliationTechnion
contact emailtamarz@technion.ac.il
dataset submitter
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Dataset FTP location
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