PXD035184

PXD035184 is an original dataset announced via ProteomeXchange.

Title	Interactome of CFTR using Apex and TurboID proximity labelling
Description	The proteins interacting with CFTR and its mutants have been intensively studied using different experimental approaches, enabling to better understand the cellular processes leading to proper protein folding, routing to the plasma membrane, recycling, activation and degradation. Recently, several strategies have been developed based on the proximity labelling of protein partners or proteins in close vicinity and their subsequent identification by mass spectrometry. In this study, we evaluate TurboID and APEX2 proximity labelling approaches on WT CFTR and compare results to those obtained by co-immunoprecipitation and in databases. We then compared the CFTR-WT interactome to two mutants of CFTR (G551D and W1282X) and the structurally unrelated potassium channel KCNK3. The two methods efficiently identified both known and novel CFTR protein partners and in proximity such as multiple SLC transporters. Hence proximity labelling approaches enable to obtain a wider picture of the CFTR interactome, feeding databases and giving a better understanding of the CFTR environment.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:29:33.418.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chiara guerrera
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-08 05:43:55	ID requested
1	2022-10-14 00:56:55	announced
⏵ 2	2023-11-14 08:29:34	announced	2023-11-14: Updated project metadata.

Chevalier B, Baatallah N, Najm M, Castanier S, Jung V, Pranke I, Golec A, Stoven V, Marullo S, Antigny F, Guerrera IC, Sermet-Gaudelus I, Edelman A, Hinzpeter A, Differential CFTR-Interactome Proximity Labeling Procedures Identify Enrichment in Multiple SLC Transporters. Int J Mol Sci, 23(16):(2022) [pubmed]

submitter keyword: Apex, TurboID, KCNK3,CFTR, interactome

Chiara Guerrera
contact affiliation	INSERM US24/CNRS UAR3633, Proteomic Platform Necker, University of Paris Cité—Federative Research Structure Necker, Paris, 75015 France
contact email	chiara.guerrera@inserm.fr
lab head
Chiara guerrera
contact affiliation	Necker proteomics, INSERM
contact email	chiara.guerrera@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD035184

PRIDE project URI

• PRIDE
  1. PXD035184
     1. Label: PRIDE project
     2. Name: Interactome of CFTR using Apex and TurboID proximity labelling