PXD035183

PXD035183 is an original dataset announced via ProteomeXchange.

Title	LiP-MS on HEK293 lysate treated with rapamycin
Description	Limited proteolysis coupled to mass spectrometry (LiP-MS) is a structural proteomics technique that can be used to identify the targets of small molecules. This is achieved by incubating lysate with the drug of interest, which induces structural changes such as occupation of the binding site or changes and protein-protein interactions. This is followed by a limited proteolysis step in which the unspecific protease proteinase K is added to the treated lysate. The protease preferentially cleaves accessible and flexible regions of the proteins, thus generating peptides that reflect the changes in surface accessibility caused by the treatment with the small molecule. Here we treated HEK293 cell lysate with rapamycin and identify the known main target FKBP1A. This data is intended to be used as a model dataset for LiP-MS data evaluation and was created as part of the IMI EUbOPEN project.
HostingRepository	PRIDE
AnnounceDate	2023-04-26
AnnouncementXML	Submission_2023-04-26_06:41:23.674.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	VivianeReber
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-08 03:41:16	ID requested
⏵ 1	2023-04-26 06:41:24	announced

Dataset with its publication pending

submitter keyword: Human, target deconvolution, LiP-MS, structural proteomics

MatthiasGstaiger
contact affiliation	Institute of Molecular Systems Biology, ETH Zurich
contact email	matthias.gstaiger@imsb.biol.ethz.ch
lab head
VivianeReber
contact affiliation	IMSB, ETH Zurich
contact email	reberv@ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD035183

PRIDE project URI

• PRIDE
  1. PXD035183
     1. Label: PRIDE project
     2. Name: LiP-MS on HEK293 lysate treated with rapamycin