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PXD035177

PXD035177 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleArthritis protection conferred by a subset of antibodies to citrullinated proteins in rheumatoid arthritis - macrophage samples
DescriptionAnti-citrullinated protein antibodies (ACPAs) are one of the hallmarks in rheumatoid arthritis (RA), but the in vivo function remained unclear. To investigate the effects of ACPAs, we expressed monoclonal ACPAs derived from RA patients, and analyzed the functions in mouse models, as well as the reactivity to tissue and peptides/proteins. All ACPAs showed no arthritogenicity and could not induce pain-like behavior in mice whereas one of them (E4) profoundly protected against antibody-induced arthritis. E4 showed a tissue binding pattern restricted to skin epithelial cells, to macrophages and dendritic cells in lymphoid tissue and to cartilage from mouse and human arthritic joints. Proteomic analysis showed that E4 had a distinct binding pattern to macrophage and RA synovial fluid proteins (e.g. alpha-enolase). The protective effect was epitope specific but also dependent on Fc-FCGR2B interaction on macrophages following the immune complex formation, resulting an increased IL-10 production and osteoclastogenesis reduction. The findings suggest that certain ACPAs could be protective in arthritis with therapeutic potentials.
HostingRepositoryPRIDE
AnnounceDate2023-01-09
AnnouncementXMLSubmission_2023-01-09_07:08:58.385.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristianBeusch
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-07-08 01:31:18ID requested
12023-01-09 07:08:58announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: anti-citrullinated protein antibodies,rheumatoid arthritis, autoimmune
Contact List
RikardHolmdahl
contact affiliationDivision of Medical Inflammation Research, Dept. Medical Biochemistry and Biophysics, Karolinska Institute, Sweden
contact emailrikard.holmdahl@ki.se
lab head
ChristianBeusch
contact affiliationDivision of Chemistry I Department of Medical Biochemistry & Biophysics Karolinska Institutet
contact emailchristian.beusch@ki.se
dataset submitter
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Dataset FTP location
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