PXD035177 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Arthritis protection conferred by a subset of antibodies to citrullinated proteins in rheumatoid arthritis - macrophage samples |
Description | Anti-citrullinated protein antibodies (ACPAs) are one of the hallmarks in rheumatoid arthritis (RA), but the in vivo function remained unclear. To investigate the effects of ACPAs, we expressed monoclonal ACPAs derived from RA patients, and analyzed the functions in mouse models, as well as the reactivity to tissue and peptides/proteins. All ACPAs showed no arthritogenicity and could not induce pain-like behavior in mice whereas one of them (E4) profoundly protected against antibody-induced arthritis. E4 showed a tissue binding pattern restricted to skin epithelial cells, to macrophages and dendritic cells in lymphoid tissue and to cartilage from mouse and human arthritic joints. Proteomic analysis showed that E4 had a distinct binding pattern to macrophage and RA synovial fluid proteins (e.g. alpha-enolase). The protective effect was epitope specific but also dependent on Fc-FCGR2B interaction on macrophages following the immune complex formation, resulting an increased IL-10 production and osteoclastogenesis reduction. The findings suggest that certain ACPAs could be protective in arthritis with therapeutic potentials. |
HostingRepository | PRIDE |
AnnounceDate | 2023-01-09 |
AnnouncementXML | Submission_2023-01-09_07:08:58.385.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | ChristianBeusch |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-07-08 01:31:18 | ID requested | |
⏵ 1 | 2023-01-09 07:08:58 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: anti-citrullinated protein antibodies,rheumatoid arthritis, autoimmune |
Contact List
RikardHolmdahl |
contact affiliation | Division of Medical Inflammation Research, Dept. Medical Biochemistry and Biophysics, Karolinska Institute, Sweden |
contact email | rikard.holmdahl@ki.se |
lab head | |
ChristianBeusch |
contact affiliation | Division of Chemistry I Department of Medical Biochemistry & Biophysics Karolinska Institutet |
contact email | christian.beusch@ki.se |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035177
- Label: PRIDE project
- Name: Arthritis protection conferred by a subset of antibodies to citrullinated proteins in rheumatoid arthritis - macrophage samples