PXD035174

PXD035174 is an original dataset announced via ProteomeXchange.

Title	Livin is upregulated in keratinocytes and promotes the release of inflammatory mediators in psoriasis
Description	Psoriasis is a common chronic inflammatory skin disease. Keratinocytes (KCs) are important effector cells that can recruit inflammatory cells by releasing inflammatory factors and chemokines to promote the inflammatory cascade in psoriasis. However, the mechanism underlying KC activation in psoriasis remains unclear. Livin is an inhibitor of apoptotic proteins and its expression can directly affect the proliferation and metastasis of tumor cells. Livin expression has been reported to be significantly increased in the lesions of patients with psoriasis; however, its specific role in KC activation has not yet been reported. The aim of this study was to investigate whether livin regulates KC activation and causes the release of inflammatory mediators. The expression levels of livin in patients with psoriasis, an imiquimod (IMQ) mouse model, and M5-treated HaCaT cells were determined via immunofluorescence staining, reverse transcription-quantitative polymerase chain reaction, enzyme-linked immunosorbent assay (ELISA), and western blotting. We constructed livin knockdown (Knockdown-HaCaT) and negative control (NC-HaCaT) cells using human immunodeficiency virus-1-based lentiviral vectors to study the function of livin in KCs via RNA-sequencing and proteomics analysis. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed. Moreover, the effect of livin expression on the release of inflammatory mediators in KCs was verified using ELISA.
HostingRepository	PRIDE
AnnounceDate	2024-05-22
AnnouncementXML	Submission_2024-05-22_02:32:41.335.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	zeng liang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-08 01:26:27	ID requested
⏵ 1	2024-05-22 02:32:42	announced

10.1111/srt.13603;

Che D, Li Y, Hang B, Li K, Wang K, Wang H, Livin expression promotes keratinocyte release of inflammatory mediators in psoriasis. Skin Res Technol, 30(2):e13603(2024) [pubmed]

submitter keyword: keratinocytes, psoriasis, inflammatory mediators,Livin

Delu Che
contact affiliation	the Second Affiliated Hospital of Xi'an Jiaotong University
contact email	chedelu22@163.com
lab head
zeng liang
contact affiliation	SHANGHAI INSTITUTES FOR BIOLOGICAL SCIENCES
contact email	zengliang@sibs.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD035174

PRIDE project URI

• PRIDE
  1. PXD035174
     1. Label: PRIDE project
     2. Name: Livin is upregulated in keratinocytes and promotes the release of inflammatory mediators in psoriasis