PXD035152

PXD035152 is an original dataset announced via ProteomeXchange.

Title	Tumor microenvironment remodeling by cancer cell-released tracks on type I collagen
Description	Metastasis is the leading cause of cancer-related deaths. During this process, tumor cells acquire invasive and migratory capacities in order to invade the surrounding tissues. To achieve this, the tumor microenvironment is altered to facilitate cancer cells proliferation and dissemination. Multiple mechanisms are involved in metastasis formation, including cell-cell communications through the tumor microenvironment and extracellular matrix remodeling. Extracellular vesicles (EVs) such as exosomes or migrasomes are already known to induce pro-tumor features such as migration, promoting tumor development and metastasis formation. Here we describe a new type of extracellular vesicles (referred as tracks) released by cancer cells and specifically attached along collagen fibers during the cell migration. We characterized these tracks, their formation, their ultrastructure as well as their molecular composition in terms of proteins and nucleic acids (miRNA), showing that they are different from classical extracellular vesicles known so far. These tracks are promoted and identified by discoidin domain receptor 1 (DDR1) in the extracellular space. Moreover, these tracks are very stable structures and surrounding cells can internalize them. After internalization, they modify the differentiation status and the phenotype of recipient cells, promoting epithelial to mesenchymal transition, cell proliferation, matrix degradation and also invasion. These data show that these collagen-associated EVs (tracks) have a role in cell-cell communication and participate in the tumor microenvironment remodeling. Consequently, these cancer-related tracks could be a new player in the tumor invasion process.
HostingRepository	PRIDE
AnnounceDate	2026-02-24
AnnouncementXML	Submission_2026-02-24_12:56:26.083.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dupuy Jean-William
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-07 01:07:44	ID requested
⏵ 1	2026-02-24 12:56:27	announced

Rouyer L, Normand L, Richard E, Allain N, Giraud J, Di-Tommaso S, Dourthe C, Raymond AA, Dupuy JW, Moreau K, Iggo R, MacGrogan G, Dugot-Senant N, Bouter A, Tan S, Favereaux A, Moreau V, Luco RF, Ros M, Saltel F, Cancer cells transfer invasive properties through microRNAs contained in collagen tracks. Cell Rep, 44(12):116725(2025) [pubmed]

10.1016/j.celrep.2025.116725;

submitter keyword: extracellular vesicles, Type I collagen fibers, migration

Frederic Saltel
contact affiliation	BoRdeaux Institute of onCology (BRIC) UMR1312 Inserm / Université de Bordeaux Team 3 Liver cancers and invasion 146 rue Léo Saignat 33076 Bordeaux, France
contact email	frederic.saltel@inserm.fr
lab head
Dupuy Jean-William
contact affiliation	OncoProt plateform, UAR TBMCore CNRS 3427 INSERM US005 University of Bordeaux
contact email	jean-william.dupuy@u-bordeaux.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD035152

PRIDE project URI

• PRIDE
  1. PXD035152
     1. Label: PRIDE project
     2. Name: Tumor microenvironment remodeling by cancer cell-released tracks on type I collagen