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PXD035151

PXD035151 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSearch for host factors located in the vicinity of the cytoplasmic tails of the mumps virus glycoproteins
DescriptionMumps virus (MuV) is the etiological agent of mumps, a disease characterized by painful swelling of the parotid glands and often accompanied by severe complications. To understand the molecular mechanism of MuV infection, a functional analysis of the involved host factors is required. However, little is known about the host factors involved in MuV infection, especially those involved in the late stage of infection. In this project, we introduced the N-terminal and C-terminal fragments of split-TurboID into the cytoplasmic domains of hemagglutinin-neuraminidase and fusion proteins, respectively, and confirmed reconstitution and functional ligase activity of split-TurboID. Following affinity purification and mass spectrometry, 641 proteins were identified as being significantly enriched in the split-TurboID-expressing cells.
HostingRepositoryPRIDE
AnnounceDate2022-10-29
AnnouncementXMLSubmission_2022-10-28_20:49:29.905.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmittertomohisahatta
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListbiotinylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-07-07 01:07:26ID requested
12022-10-28 20:49:30announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Proximity labeling, Split-TurboID, Glycoprotein,Mumps virus
Contact List
HiroshiKatoh
contact affiliationDepartment of Virology III, National Institute of Infectious Diseases
contact emailkato0704@niid.go.jp
lab head
tomohisahatta
contact affiliationRobotic Biology Institute Inc. Scientific support team
contact emailtomohisa.hatta@rbi.co.jp
dataset submitter
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Dataset FTP location
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