PXD035151 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Search for host factors located in the vicinity of the cytoplasmic tails of the mumps virus glycoproteins |
Description | Mumps virus (MuV) is the etiological agent of mumps, a disease characterized by painful swelling of the parotid glands and often accompanied by severe complications. To understand the molecular mechanism of MuV infection, a functional analysis of the involved host factors is required. However, little is known about the host factors involved in MuV infection, especially those involved in the late stage of infection. In this project, we introduced the N-terminal and C-terminal fragments of split-TurboID into the cytoplasmic domains of hemagglutinin-neuraminidase and fusion proteins, respectively, and confirmed reconstitution and functional ligase activity of split-TurboID. Following affinity purification and mass spectrometry, 641 proteins were identified as being significantly enriched in the split-TurboID-expressing cells. |
HostingRepository | PRIDE |
AnnounceDate | 2022-10-29 |
AnnouncementXML | Submission_2022-10-28_20:49:29.905.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | tomohisahatta |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | biotinylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-07-07 01:07:26 | ID requested | |
⏵ 1 | 2022-10-28 20:49:30 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Proximity labeling, Split-TurboID, Glycoprotein,Mumps virus |
Contact List
HiroshiKatoh |
contact affiliation | Department of Virology III, National Institute of Infectious Diseases |
contact email | kato0704@niid.go.jp |
lab head | |
tomohisahatta |
contact affiliation | Robotic Biology Institute Inc. Scientific support team |
contact email | tomohisa.hatta@rbi.co.jp |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD035151 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035151
- Label: PRIDE project
- Name: Search for host factors located in the vicinity of the cytoplasmic tails of the mumps virus glycoproteins