PXD035147

PXD035147 is an original dataset announced via ProteomeXchange.

Title	Integrated proteome and malonylome analyses reveal the potential meaning of TLN1 and ACTB in end-stage renal disease
Description	Purpose: End-stage renal disease (ESRD) is a condition that is characterized by the loss of kidney function. ESRD patients suffer from various endothelial dysfunctions, inflammation, and immune system defects. Lysine malonylation (Kmal) is a recently discovered post-translational modification (PTM). Although Kmal has the ability to regulate a wide range of biological processes in various organisms, its specific role in ESRD is limited. Experimental design: In this study, the affinity enrichment and liquid chromatography-tandem mass spectrometry (LC-MS/MS) techniques have been used to create the first global proteome and malonyl proteome (malonylome) profiles of peripheral blood mononuclear cells (PBMCs) from twenty patients with ESRD and eighty-one controls. Results: On analysis, 793 differentially expressed proteins (DEPs) and 12 differentially malonylated proteins (DMPs) with 16 Kmal sites were identified. The Rap1 signaling pathway and platelet activation pathway were found to be important in the development of chronic kidney disease (CKD), as were DMPs TLN1 and ACTB, as well as one malonylated site. One conserved Kmal motif was also discovered. Conclusion: These findings provided the first report on the Kmal profile in ESRD, which could be useful in understanding the potential role of lysine malonylation modification in the development of ESRD.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:06:52.280.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ruqi tan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-07 01:02:46	ID requested
1	2023-10-24 10:59:25	announced
⏵ 2	2023-11-14 09:07:00	announced	2023-11-14: Updated project metadata.

Tan R, Li D, Hu N, Qiu J, Zeng Z, Cai W, Zhong Y, Zhang X, Pai P, Wang K, Tang D, Dai Y, Integrated proteome and malonylome analyses reveal the potential meaning of TLN1 and ACTB in end-stage renal disease. Proteome Sci, 21(1):18(2023) [pubmed]

submitter keyword: lysine malonylation (Kmal), platelet activation, post-translational modification (PTM), end-stage renal disease (ESRD), chronic kidney disease (CKD)

Ruqi Tan
contact affiliation	Clinical Medical Research Center, The Second Clinical Medical College of Jinan University (Shenzhen People’s Hospital), Shenzhen, Guangdong;Department of Nephrology, The University of Hong Kong-Shenzhen Hospital, Shenzhen, Guangdong
contact email	674909173@qq.com
lab head
ruqi tan
contact affiliation	Clinical Medical Research Center, The Second Clinical Medical College of Jinan University (Shenzhen People’s Hospital), Shenzhen, Guangdong;Department of Nephrology, The University of Hong Kong-Shenzhen Hospital, Shenzhen, Guangdong
contact email	674909173@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035147
     1. Label: PRIDE project
     2. Name: Integrated proteome and malonylome analyses reveal the potential meaning of TLN1 and ACTB in end-stage renal disease