PXD035124

PXD035124 is an original dataset announced via ProteomeXchange.

Title	Directed growth and fusion of membrane-wall microdomains requires CASP-mediated inhibition and displacement of secretory foci
Description	Casparian strips (CS), the main extracellular diffusion barrier in plant roots, are precisely localized cell wall lignin-impregnations, contrasting animal tight-junctions. The CS membrane domain (CSD) proteins 1-5 (CASP1-5) define and accumulate at the CS associated membrane domains displaying matrix adhesion and protein exclusion. A full CASP knock-out (caspQ) now reveals that CASPs are not needed for localization of lignification or lignin-polymerizing enzymes, since correctly aligned spots still form in the mutant. Ultra-structurally, however, these spots appear as highly disorganized secretory foci, with neither exclusion zone nor membrane attachment and excessive cell wall growth. Biotin proximity labelling identifies RabA-GTPases as potential CASP-interactors. We confirm their localisation and function at the CSD, similar to exocyst subunits, known Rab effectors. Our work reveals that CASPs enforce displacement of initial secretory foci through exclusion of vesicle tethering factors, thereby ensuring rapid fusion of microdomains and effective sealing of the cell wall space.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:46:33.931.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Patrice Waridel
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-06 14:35:45	ID requested
1	2023-05-10 13:22:53	announced
⏵ 2	2023-11-14 08:46:40	announced	2023-11-14: Updated project metadata.

Barbosa ICR, De Bellis D, Fl, ü, ckiger I, Bellani E, Grang, é, -Guerment M, H, é, maty K, Geldner N, Directed growth and fusion of membrane-wall microdomains requires CASP-mediated inhibition and displacement of secretory foci. Nat Commun, 14(1):1626(2023) [pubmed]

submitter keyword: Arabidopsis thaliana, TurboID, Fusion, CASP

Niko Geldner
contact affiliation	University of Lausanne, Faculty of Biology and Medicine, Department of Plant Molecular Biology, 1015 Lausanne, Switzerland
contact email	niko.geldner@unil.ch
lab head
Patrice Waridel
contact affiliation	University of Lausanne
contact email	patrice.waridel@unil.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035124
     1. Label: PRIDE project
     2. Name: Directed growth and fusion of membrane-wall microdomains requires CASP-mediated inhibition and displacement of secretory foci