PXD035112

PXD035112 is an original dataset announced via ProteomeXchange.

Title	Identification of Biomarkers and Therapeutic Targets Related to Sepsis-associated Encephalopathy in Rats by Quantitative Proteomics
Description	Background: Sepsis-associated encephalopathy (SAE) is a common and severe complication of sepsis. While several studies have reported the proteomic alteration in plasma, urine, heart, etc. of sepsis, few research focused on the brain tissue. This study aims at discovering the differentially abundant proteins in the brains of septic rats to identify biomarkers of SAE. Methods: The Prague-Dawley rats were randomly divided into sepsis (n = 6) or sham (n = 6) groups, and then the whole brain tissue was dissected at 24 h after surgery for further protein identification by TMT-LC–MS/MS-based proteomics. Ingenuity pathway analysis, Gene ontology knowledgebase, and STRING database are used to explore the biological significance of proteins with altered concentration. Results: Among the total of 3163 proteins identified in the brain tissue, 57 were increased while 38 were decreased in the sepsis group compared to the sham group. Bioinformatic analyses suggest that the differentially abundant proteins are highly related to cellular microtubule metabolism, energy production, nucleic acid metabolism, neurological disease, etc. Additionally, acute phase response signaling was possibly activated and PI3K/AKT signaling was suppressed during sepsis. An interaction network established by IPA revealed that Akt1, Gc-globulin, and ApoA1 were the core proteins. The increase of Gc-globulin and the decrease of Akt1 were confirmed by Western blot. Conclusions: Based on the multifunction of these proteins in several brain diseases, we first propose that Gc-globulin, ApoA1, PI3K/AKT pathway, and acute phase response proteins (hemopexin and cluster of alpha-2-macroglobulin) can potentially be diagnosis biomarkers, therapeutic targets, and prognosis indicators of SAE. These results may provide new insights into the pathologic mechanism of SAE, yet further research is required to explore the functional implications and clinical applications of the differentially abundant proteins in the brains of sepsis group.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:51:33.287.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	miaoxian yang
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-06 14:21:03	ID requested
1	2023-03-10 13:00:09	announced
⏵ 2	2023-11-14 07:51:35	announced	2023-11-14: Updated project metadata.

Yang M, He Y, Xin Y, Jiang J, Tian M, Tan J, Deng S, Gong Y, Identification of biomarkers and therapeutic targets related to Sepsis-associated encephalopathy in rats by quantitative proteomics. BMC Genomics, 24(1):4(2023) [pubmed]

submitter keyword: Quantitative proteomics, PI3K/AKT signaling, Acute phase response,Sepsis-associated encephalopathy, GC

yang miaoxian
contact affiliation	Huashan Hospital, Fudan University
contact email	youngmiaoxian@163.com
lab head
miaoxian yang
contact affiliation	Department of Critical Care Medicine, Huashan Hospital, Fudan University
contact email	youngmiaoxian@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035112
     1. Label: PRIDE project
     2. Name: Identification of Biomarkers and Therapeutic Targets Related to Sepsis-associated Encephalopathy in Rats by Quantitative Proteomics