PXD035110

PXD035110 is an original dataset announced via ProteomeXchange.

Title	Vap33 protein interactome - The Drosophila tumor suppressors Lgl and Vap33 activate the Hippo pathway by a dual mechanism involving RtGEF/Git/Arf79F and inhibition of the V-ATPase
Description	To identify Vap33 binding proteins, we purified Vap33-containing protein complexes from Drosophila embryos, using the single-step GFP-trap bead purification from a fly line expressing YFP-tagged Vap33, followed by nanoLC-MS/MS analysis of the interactors. This study revealed the binding of Vap33 to RtGEF and Git, which we further connected to the regulation of Hippo signaling.
HostingRepository	PRIDE
AnnounceDate	2024-02-01
AnnouncementXML	Submission_2024-02-01_12:12:01.341.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexey Veraksa
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	iodoacetic acid derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-06 14:12:16	ID requested
⏵ 1	2024-02-01 12:12:02	announced

10.1242/jcs.261917;

Portela M, Mukherjee S, Paul S, La Marca JE, Parsons LM, Veraksa A, Richardson HE, The Drosophila tumour suppressor Lgl and Vap33 activate the Hippo pathway through a dual mechanism. J Cell Sci, 137(4):(2024) [pubmed]

submitter keyword: Vap33, embryo,Drosophila

Alexey Veraksa
contact affiliation	Biology Department University of Massachusetts Boston Boston, USA
contact email	alexey.veraksa@umb.edu
lab head
Alexey Veraksa
contact affiliation	University of Massachusetts Boston
contact email	alexey.veraksa@umb.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035110
     1. Label: PRIDE project
     2. Name: Vap33 protein interactome - The Drosophila tumor suppressors Lgl and Vap33 activate the Hippo pathway by a dual mechanism involving RtGEF/Git/Arf79F and inhibition of the V-ATPase