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PXD035104

PXD035104 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleUnusual composition of the mitochondrial pyruvate dehydrogenase complex from the widespread marine protist group diplonemids
DescriptionIn eukaryotes, pyruvate, a key metabolite produced by glycolysis, is converted by a mitochondrial pyruvate dehydrogenase complex (PDH) to acetyl-coenzyme A, which is fed into the tricarboxylic acid cycle. Two additional enzyme complexes catalyze similar oxidative decarboxylation reactions albeit using different substrates, the branched-chain ketoacid dehydrogenase (BCKDH) and the 2-oxoglutarate dehydrogenase (OGDH). In diplonemids, one of the most abundant and diverse groups of oceanic protists, comparative transcriptome analyses indicated that their PDH complex is compositionally unique, with the conventional E1 subunit replaced by an aceE protein of prokaryotic origin. Here we demonstrate in the model diplonemid Paradiplonema papillatum that the pyruvate dehydrogenase activity is comparable to that in other euglenozoan protists. By protein mass spectrometry we revealed that the aceE protein is twice as abundant as the E1 subunits of OGDH and enriched in the mitochondrion to the same level as the BCKDH E1 subunits, corroborating the functional relevance of the proposed aceE subunit of the PDH complex. Importantly, the acquisition of the archaeal aceE by the diplonemid ancestor led not only to the complete loss of the eukaryotic-type E1 of the PDH complex, but also its dedicated E2 and E3 subunits, still present in other euglenozoans. Hence, to reconstitute the diplonemid PDH, the aceE protein needs to partner with E2 and E3 subunits of BCKDH and/or OGDH. The diplonemid example illustrates that the acquisition and successful integration of a foreign E1p can profoundly reorganize the entire PDH complex.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_06:46:26.896.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD035104
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMaksym Danchenko
SpeciesList scientific name: Diplonema papillatum; NCBI TaxID: 91374;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-07-06 14:10:01ID requested
12023-07-28 01:59:53announced
22023-11-14 06:46:27announced2023-11-14: Updated project metadata.
Publication List
10.6019/PXD035104;
Keyword List
submitter keyword: Paradiplonema papillatum, 2-oxoglutarate dehydrogenase, branched-chain ketoacid dehydrogenase
Contact List
Peter BarĂ¡th
contact affiliationDepartment of Glycobiology, Institute of Chemistry, Slovak Academy of Sciences
contact emailpeter.barath@savba.sk
lab head
Maksym Danchenko
contact affiliationPlant Science and Biodiversity Center SAS
contact emailmaxymdan@gmail.com
dataset submitter
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