PXD035080

PXD035080 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of the secretome of selected Mycobacterium tuberculosis Erdman strains
Description	Extracellular Mycobacterium tuberculosis (Mtb) aggregates can evade phagocytosis and intracellular host-cell defenses by inducing macrophage killing. We showed that Mtb aggregates require a functional ESX-1 type VII secretion system and production of the surface-exposed lipid phthiocerol dimycocerosate (PDIM) to induce cell death upon contact with macrophages. We used quantitative proteomics to compare the secretome of a panel of Mtb mutant strains that induce or do not induce macrophage death and identified secreted bacteria factors involved in macrophage death induction.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:57:35.754.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chiara Toniolo
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Mycobacterium tuberculosis str. Erdman = ATCC 35801; NCBI TaxID: 652616;
ModificationList	phosphorylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-04 15:00:10	ID requested
1	2023-03-16 06:28:31	announced
⏵ 2	2023-11-14 08:57:36	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Mycobacterium tuberculosis, ESX-1 secretion system, secretome

John McKinney
contact affiliation	School of Life Sciences, Swiss Federal Institute of Technology in Lausanne (EPFL)
contact email	john.mckinney@epfl.ch
lab head
Chiara Toniolo
contact affiliation	Ecole Polotechnique Federale de Lausanne (EPFL), SV, GHI, UPKIN
contact email	chiara.toniolo87@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD035080

PRIDE project URI

• PRIDE
  1. PXD035080
     1. Label: PRIDE project
     2. Name: Proteomic analysis of the secretome of selected Mycobacterium tuberculosis Erdman strains