PXD035073

PXD035073 is an original dataset announced via ProteomeXchange.

Title	comparative proteomics and elemental analysis reveal molecular adaption of Mollusca radula to different lifestyles
Description	Radula is a unique foraging organ to Mollusca, which is important for their evolution and taxonomic classification. Many radulae are mineralized with metals. Although the remarkable mechanical properties of mineralized radula are well-studied, the formation of mineralization from nonmineralized radula is poorly understood. Taking advantage of the recently sequenced octopus and chiton genome, we were able to identify more species-specific radula proteins by proteomics. Comparing these proteomes enable us to gain insight into the molecular components of nonmineralized and mineralized radula, highlighting that iron mineralization in chiton radula is possibly due to the evolution of ferritins and peroxiredoxins. Through in vitro binding assay, ferritin is shown to be important to iron accumulation into the nonmineralized radula. Moreover, radula proteomes are well adapted to their functionality. Octopus radula has many scaffold modification proteins to suit flexibility while chiton radula has abundant sugar metabolism proteins (e.g. glycosyl hydrolases) to adapt to algae feeding. This study provides a foundation for the understanding of Mollusca radula formation and evolution and may inspire the synthesis of iron nanomaterials.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:14:31.636.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chuang Liu
SpeciesList	scientific name: Acyrthosiphon pisum (Pea aphid); NCBI TaxID: 7029;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-04 14:51:29	ID requested
1	2022-10-27 03:28:20	announced
⏵ 2	2023-11-14 08:14:32	announced	2023-11-14: Updated project metadata.

Liu C, Sun D, Chen Y, Wang C, Li J, Lin J, Mineralize It or Not: Comparative Proteomics and Elemental Analysis Reveal Ancestral Compositions of Iron Mineralized Molluscan Radulae. J Proteome Res, 21(11):2736-2742(2022) [pubmed]

submitter keyword: radula

Chuang Liu
contact affiliation	Hohai University
contact email	helloliuchuang@163.com
lab head
Chuang Liu
contact affiliation	Hohai University, Nanjing, China
contact email	helloliuchuang@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD035073

PRIDE project URI

• PRIDE
  1. PXD035073
     1. Label: PRIDE project
     2. Name: comparative proteomics and elemental analysis reveal molecular adaption of Mollusca radula to different lifestyles