PXD035062

PXD035062 is an original dataset announced via ProteomeXchange.

Title	Dynamic of chromosome-binding protein complexes to meiotic prophase I of mouse spermatocyte
Description	Meiotic prophase I (MPI) is the most important event in mammalian meiosis. Even though MPI-related gene expression has been extensively investigated, the status of the chromosome binding proteins (CBPs) and the corresponding complexes in this process has been not scrutinized yet. A quantitative proteomics focused onto the MPI-related CBPs was accomplished in this study, in which the mouse primary spermatocytes in 4 different subphases of MPI were collected and the chromosome-enriched proteins (ChEPs) within the cells were extracted and identified in quantitative mode. According to a stringent criterion, in total of 1136 CBPs in the MPI subphases were identified and quantified. Looking at the dynamic patterns of CBP abundance in response to MPI progression, the patterns were broadly divided to two groups, high abundance in leptotene (L) and zygotene (Z) or that in pachytene (P) and diplotene (D). Most importantly, 26 CBP complexes were found, while some MPI-dependent features of these complexes were perceived for the first time. The functions of these complexes were categorized to DNA replication, pre-initial complex of transcription, transcriptional regulation and synapsis. In agreement with the current theory, the dynamic changes of the CBP complex abundance appeared declined DNA replication and activated transcription and synapsis activities in P and D, and Z and P phase; in converse to the traditional prediction, the abundance response of CBP complexes to MPI emerged condensin activity weakened in P and D phase. Profiling of protein complexes thus offered convincing evidence how important the CBP complexes partake in MPI regulation.
HostingRepository	iProX
AnnounceDate	2023-06-08
AnnouncementXML	Submission_2023-06-09_00:10:16.780.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hongkai Xu
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-03 23:57:15	ID requested
⏵ 1	2023-06-09 00:10:17	announced

Zhang Y, Yang L, Fang K, Li Q, Xu H, Ren Y, Zi J, Chen CD, Liu S, Dynamic Responses of Chromosome-Binding Protein Complexes to Meiotic Prophase I of Mouse Spermatocyte. J Proteome Res, 21(11):2715-2726(2022) [pubmed]

submitter keyword: meiosis, quantitative proteomics, chromosome, protein complexes

Siqi Liu
contact affiliation	BGI-Shenzhen
contact email	siqiliu@genomics.cn
lab head
Hongkai Xu
contact affiliation	BGI-Shenzhen
contact email	xuhongkai@genomics.cn
dataset submitter

iProX dataset URI