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PXD035060

PXD035060 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleData on the change in the protein profiles of human subcutaneous white adipocytes during differentiation and maturation
DescriptionWe analyzed changes in the protein profiles that occur during differentiation and maturation of cultured human subcutaneous white preadipocytes. We divided the three cell lines (Cell Line-1, Cell Line-2, and Cell Line-3) of Caucasian-derived subcutaneous preadipocytes into five stages: i) subcutaneous preadipocytes as stage-1, ii) after inducing differentiation into adipocytes as stage-2, iii-v) from the initiation of lipid droplet formation to become mature subcutaneous adipocytes was defined as stage-3 to stage-5, depending on lipid droplet amount and formation. Proteins from the cells were extracted at each stage (stage-1 to stage-5), proteolytically cleaved using trypsin, and analyzed using untargeted liquid chromatography and mass spectrometry.
HostingRepositoryjPOST
AnnounceDate2022-07-04
AnnouncementXMLSubmission_2023-04-05_00:40:25.757.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTakuya kitamoto
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-07-03 22:16:41ID requested
12022-07-03 22:16:44announced
22022-09-18 03:50:04announced2022-09-18: Updated FTP location.
32023-04-05 00:40:26announced2023-04-05: Updated PubMed.
Publication List
Kitamoto T, Kitamoto A, Updating "Data on changes in lipid profiles during the differentiation and maturation of human subcutaneous white adipocytes analyzed using chromatographic and bioinformatic tools" with data on the changes in protein profiles. Data Brief, 48():109036(2023) [pubmed]
Keyword List
submitter keyword: Subcutaneous preadipocytes, Subcutaneous adipocytes, Proteomics, Mass spectrometry
Contact List
Aya Kitamoto
lab head
Takuya kitamoto
contact affiliationHamamatsu University School of Medicine
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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