PXD035043
PXD035043 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Paradiplonema papillatum E3 interactome |
Description | In eukaryotes, pyruvate, a key metabolite produced by glycolysis, is converted by a mitochondrial pyruvate dehydrogenase complex (PDH) to acetyl-coenzyme A, which is fed into the tricarboxylic acid cycle. Two additional enzyme complexes catalyze similar oxidative decarboxylation reactions albeit using different substrates, the branched-chain ketoacid dehydrogenase (BCKDH) and the 2-oxoglutarate dehydrogenase (OGDH). In diplonemids, one of the most abundant and diverse groups of oceanic protists, comparative transcriptome analyses indicated that their PDH complex is compositionally unique, with the conventional E1 subunit replaced by an aceE protein of prokaryotic origin. Here we endogenously tagged the common E3 subunit with a protein A tag and performed immunoprecipitation experiments. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:54:01.821.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Corinna Benz |
SpeciesList | scientific name: Diplonema papillatum; NCBI TaxID: 91374; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-06-30 07:34:44 | ID requested | |
1 | 2023-07-16 23:13:58 | announced | |
2 | 2023-11-14 09:02:17 | announced | 2023-11-14: Updated project metadata. |
⏵ 3 | 2024-10-22 05:54:02 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1016/j.bbagen.2023.130419; |
Z, á, honov, á K, Valach M, Tripathi P, Benz C, Opperdoes FR, Barath P, Luk, á, č, ov, á V, Danchenko M, Faktorov, á D, Horv, á, th A, Burger G, Luke, š J, Š, kodov, á, -Sver, á, kov, á I, Subunit composition of mitochondrial dehydrogenase complexes in diplonemid flagellates. Biochim Biophys Acta Gen Subj, 1867(9):130419(2023) [pubmed] |
Keyword List
submitter keyword: Paradiplonema papillatum, immunoprecipitation, PDH complex |
Contact List
Julius Lukes | |
---|---|
contact affiliation | Institute of Parasitology Biology Center, Czech Academy of Sciences Branišovská 31 370 05 České Budějovice |
contact email | jula@paru.cas.cz |
lab head | |
Corinna Benz | |
contact affiliation | Institute of Parasitology Biology Center, Czech Academy of Sciences Branišovská 31 370 05 České Budějovice |
contact email | corinna.benz@paru.cas.cz |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD035043 |
PRIDE project URI |
Repository Record List
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