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PXD035043

PXD035043 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleParadiplonema papillatum E3 interactome
DescriptionIn eukaryotes, pyruvate, a key metabolite produced by glycolysis, is converted by a mitochondrial pyruvate dehydrogenase complex (PDH) to acetyl-coenzyme A, which is fed into the tricarboxylic acid cycle. Two additional enzyme complexes catalyze similar oxidative decarboxylation reactions albeit using different substrates, the branched-chain ketoacid dehydrogenase (BCKDH) and the 2-oxoglutarate dehydrogenase (OGDH). In diplonemids, one of the most abundant and diverse groups of oceanic protists, comparative transcriptome analyses indicated that their PDH complex is compositionally unique, with the conventional E1 subunit replaced by an aceE protein of prokaryotic origin. Here we endogenously tagged the common E3 subunit with a protein A tag and performed immunoprecipitation experiments.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:54:01.821.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterCorinna Benz
SpeciesList scientific name: Diplonema papillatum; NCBI TaxID: 91374;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-30 07:34:44ID requested
12023-07-16 23:13:58announced
22023-11-14 09:02:17announced2023-11-14: Updated project metadata.
32024-10-22 05:54:02announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.bbagen.2023.130419;
Z, á, honov, á K, Valach M, Tripathi P, Benz C, Opperdoes FR, Barath P, Luk, á, č, ov, á V, Danchenko M, Faktorov, á D, Horv, á, th A, Burger G, Luke, š J, Š, kodov, á, -Sver, á, kov, á I, Subunit composition of mitochondrial dehydrogenase complexes in diplonemid flagellates. Biochim Biophys Acta Gen Subj, 1867(9):130419(2023) [pubmed]
Keyword List
submitter keyword: Paradiplonema papillatum, immunoprecipitation, PDH complex
Contact List
Julius Lukes
contact affiliationInstitute of Parasitology Biology Center, Czech Academy of Sciences Branišovská 31 370 05 České Budějovice
contact emailjula@paru.cas.cz
lab head
Corinna Benz
contact affiliationInstitute of Parasitology Biology Center, Czech Academy of Sciences Branišovská 31 370 05 České Budějovice
contact emailcorinna.benz@paru.cas.cz
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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