PXD035043

PXD035043 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Paradiplonema papillatum E3 interactome
Description	In eukaryotes, pyruvate, a key metabolite produced by glycolysis, is converted by a mitochondrial pyruvate dehydrogenase complex (PDH) to acetyl-coenzyme A, which is fed into the tricarboxylic acid cycle. Two additional enzyme complexes catalyze similar oxidative decarboxylation reactions albeit using different substrates, the branched-chain ketoacid dehydrogenase (BCKDH) and the 2-oxoglutarate dehydrogenase (OGDH). In diplonemids, one of the most abundant and diverse groups of oceanic protists, comparative transcriptome analyses indicated that their PDH complex is compositionally unique, with the conventional E1 subunit replaced by an aceE protein of prokaryotic origin. Here we endogenously tagged the common E3 subunit with a protein A tag and performed immunoprecipitation experiments.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:54:01.821.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Corinna Benz
SpeciesList	scientific name: Diplonema papillatum; NCBI TaxID: 91374;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-30 07:34:44	ID requested
1	2023-07-16 23:13:58	announced
2	2023-11-14 09:02:17	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:54:02	announced	2024-10-22: Updated project metadata.

Publication List

10.1016/j.bbagen.2023.130419;
Z, á, honov, á K, Valach M, Tripathi P, Benz C, Opperdoes FR, Barath P, Luk, á, č, ov, á V, Danchenko M, Faktorov, á D, Horv, á, th A, Burger G, Luke, š J, Š, kodov, á, -Sver, á, kov, á I, Subunit composition of mitochondrial dehydrogenase complexes in diplonemid flagellates. Biochim Biophys Acta Gen Subj, 1867(9):130419(2023) [pubmed]

10.1016/j.bbagen.2023.130419;

Z, á, honov, á K, Valach M, Tripathi P, Benz C, Opperdoes FR, Barath P, Luk, á, č, ov, á V, Danchenko M, Faktorov, á D, Horv, á, th A, Burger G, Luke, š J, Š, kodov, á, -Sver, á, kov, á I, Subunit composition of mitochondrial dehydrogenase complexes in diplonemid flagellates. Biochim Biophys Acta Gen Subj, 1867(9):130419(2023) [pubmed]

Keyword List

submitter keyword: Paradiplonema papillatum, immunoprecipitation, PDH complex

submitter keyword: Paradiplonema papillatum, immunoprecipitation, PDH complex

Contact List

Julius Lukes
contact affiliation	Institute of Parasitology Biology Center, Czech Academy of Sciences Branišovská 31 370 05 České Budějovice
contact email	jula@paru.cas.cz
lab head
Corinna Benz
contact affiliation	Institute of Parasitology Biology Center, Czech Academy of Sciences Branišovská 31 370 05 České Budějovice
contact email	corinna.benz@paru.cas.cz
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD035043
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD035043

PRIDE project URI

Repository Record List

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