PXD035014

PXD035014 is an original dataset announced via ProteomeXchange.

Title	A molecular insight into fluoride toxicity in skeletal muscle using invitro C2C12 and in vivo C57BL/6 mice models
Description	Fluoride toxicity in multiple organs has been extensively reported in several decades of research. In-depth study coverage is available on teeth and bone tissues. But studies addressing skeletal muscle fluorosis are scanty. C57BL/6 mice were provided with sodium fluoride in drinking water for 60 days. Histological analysis, primary culture of skeletal muscle was performed. Protein expressions were analyzed by Immunocytochemistry, qRT-PCR, and western blotting techniques. Proteomic approach was considered to overview the entire proteome response. Exposure to sodium fluoride resulted in the loss of body weight in C57BL/6 mice. The compactness of the myofibre arrangement was distorted due to the treatment. Major reduction of contractile proteins such as actinin, troponin, and myosin further loss of mitochondrial proteins were confirmed by proteomic approach. Sodium fluoride treatment altered mitochondrial function. Further, loss of contractile proteins triggered skeletal muscle weakness.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:31:36.586.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Apoorva H Nagendra
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-30 06:51:58	ID requested
1	2023-03-10 16:26:29	announced
2	2023-11-14 08:08:37	announced	2023-11-14: Updated project metadata.
⏵ 3	2023-11-14 08:31:39	announced	2023-11-14: Updated project metadata.

Nagendra AH, Najar MA, Bose B, Shenoy PS, High concentration of sodium fluoride in drinking water induce hypertrophy versus atrophy in mouse skeletal muscle via modulation of sarcomeric proteins. J Hazard Mater, 432():128654(2022) [pubmed]

Nagendra AH, Ray A, Chaudhury D, Mitra A, Ranade AV, Bose B, Shenoy P S, Sodium fluoride induces skeletal muscle atrophy via changes in mitochondrial and sarcomeric proteomes. PLoS One, 17(12):e0279261(2022) [pubmed]

submitter keyword: Mus musculus, Skeletal muscle,Sodium fluoride, Orbitrap, LC-MS/MS

Sudheer Shenoy P
contact affiliation	Associate Professor, Stem Cells and Regenerative Medicine Centre, Yenepoya Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangalore, Karnataka (State),India (C)
contact email	shenoy@yenepoya.edu.in
lab head
Apoorva H Nagendra
contact affiliation	Ph.D. Scholar
contact email	apoorva2609@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD035014

PRIDE project URI

• PRIDE
  1. PXD035014
     1. Label: PRIDE project
     2. Name: A molecular insight into fluoride toxicity in skeletal muscle using invitro C2C12 and in vivo C57BL/6 mice models