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PXD035010

PXD035010 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLow dose rate radiation induced secretion of TGF-β3 together 2 with an activator in small extracellular vesicles modifies low 3 dose hyper-radiosensitivity through ALK1 binding
DescriptionHyper-radiosensitivity (HRS) is the increased sensitivity to low doses of ionizing radiation 12 observed in most cell lines. We previously demonstrated that HRS is permanently abolished in cells irradiated at a low dose rate (LDR), in a mechanism dependent on transforming growth factor β3 (TGF-β3). In this study, we aimed to elucidate the activation and receptor binding of TGF-β3 in this mechanism. T-47D cells were pre-treated with inhibitors of potential receptors and activators of TGF-β3, along with addition of small extracellular vesicles (sEVs) from LDR primed cells, before their radiosensitivity was assessed by the clonogenic assay. The protein content of sEVs from LDR primed cells was analyzed with mass spectrometry. Our results show that sEVs contain TGF-β3 regardless of priming status, but only sEVs from LDR primed cells remove HRS in reporter cells. Inhibition of the matrix metalloproteinase (MMP) family prevents removal of HRS, suggesting an MMP-dependent activation of TGF-β3 in the LDR primed cells. We demonstrate a functional interaction between TGF-β3 and activin receptor like kinase 1 (ALK1), by showing that TGF-β3 removes HRS through ALK1 binding, independent of ALK5 and TGF-βRII. These results are an important contribution to a more comprehensive understanding of the mechanism behind TGF-β3 mediated removal of HRS.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:38:58.141.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD035010
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterBernd Thiede
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidoethyl-L-cysteine; monohydroxylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-30 03:29:15ID requested
12022-08-11 20:59:05announced
22024-10-22 05:38:59announced2024-10-22: Updated project metadata.
Publication List
10.6019/PXD035010;
10.3390/ijms23158147;
Hanson I, Pitman KE, Altanerova U, Altaner Č, Malinen E, Edin NFJ, 3 Together with an Activator in Small Extracellular Vesicles Modifies Low-Dose Hyper-Radiosensitivity through ALK1 Binding. Int J Mol Sci, 23(15):(2022) [pubmed]
Keyword List
submitter keyword: ADAM
Adaptive response
ALK1
ALK5
Bystander effects
Hyper-radiosensi-27 tive response
Low-dose radiation
Low dose rate
MMP
TGF-β3
Contact List
Ingunn Hanson
contact affiliationUniversity of Oslo, Department of Physics
contact emailIngunn.Hanson@fys.uio.no
lab head
Bernd Thiede
contact affiliationUniversity of Oslo
contact emailbernd.thiede@ibv.uio.no
dataset submitter
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Dataset FTP location
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PRIDE project URI
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