PXD035004

PXD035004 is an original dataset announced via ProteomeXchange.

Title	Mutations in the postsynaptic signaling hub TNIK disrupts neuronal progenitor cells and immature neuronal function in human models of neurodevelopmental disease
Description	Synaptic proteins, in particular those connected in large protein interaction networks at the postsynaptic site of glutamatergic neurons have been associated to a number of neurodevelopmental disorders. While their contribution to disease has been well documented in mature rodent synapses, their role in early neuronal development and in non-neuronal cell types is not known. Additionally, it is unclear if they are associated in the same protein interaction networks (PINs) in early neuronal development, particularly in human models of neurodevelopmental disease. Here we use the postsynaptic density (PSD) protein TNIK as a model of a classical PSD signaling hub associated to neurodevelopmental disease. Using hiPSC models of TNIK disfunction and disease, we show that PSD PINs are dysregulated by TNIK in immature neurons, synapses and neuronal progenitor cells (NPCs). TNIK impairs NPCs and human immature synapses using different signaling mechanisms and associating to different sets of “PSD proteins”.
HostingRepository	PRIDE
AnnounceDate	2024-03-26
AnnouncementXML	Submission_2024-03-26_09:14:13.442.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	marcelo coba
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-30 03:17:01	ID requested
⏵ 1	2024-03-26 09:14:13	announced

Dataset with its publication pending

submitter keyword: neurodevelopment,hiPSC, signaling networks, disease, postsynaptic, signaling

Marcelo Coba
contact affiliation	Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California
contact email	coba@usc.edu
lab head
marcelo coba
contact affiliation	USC
contact email	coba@usc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035004
     1. Label: PRIDE project
     2. Name: Mutations in the postsynaptic signaling hub TNIK disrupts neuronal progenitor cells and immature neuronal function in human models of neurodevelopmental disease