PXD034998

PXD034998 is an original dataset announced via ProteomeXchange.

Title	bioprospecting for fungal enzymes for applications in microalgal-biomass based biorefineries
Description	Microalgal biomass is a promising feedstock for biofuels, feed/food and biomaterials. However, while production and commercialization of single-product commodities is still not economically viable, obtaining multiple products in a biomass biorefinery faces several techno- economic challenges. The aim of this study was to identify a suitable source of hydrolytic enzymes for algal biomass saccharification. Screening of twenty-six fungal isolates for secreted enzymes activity on Chlamydomonas reinhardtii biomass resulted in the identification of Aspergillus niger IB-34 as a candidate strain. Solid state fermentation on wheat bran produced the most active enzyme preparations. From sixty-five proteins identified by LC-MS, the majority corresponded to predicted secreted proteins belonging to the Gene Ontology categories of catalytic activity/hydrolase activity on glycosyl and O-glycosyl compounds. Defatted biomass of the more biotechnologically relevant strains towards the production of commodities, Chlorella sorokiniana and Scenedesmus obliquus, was fully saccharified after a mild pretreatment at 80 °C for 10 min, at a high biomass load of 10 % (w/v). Deffated and 2 saccharified biomass of both strains was further converted into ethanol by fermentation with Saccharomyces cerevisiae at a theoretical maximum efficiency, either by separated or simultaneous sccharification and fermentation. The resulting insoluble protein after biomass defatting with an organic solvent and enzymatic saccharification resulted in a high digestibility in an in vitro digestion assay. Proof-of-concept is presented for an enzyme-assisted biomass biorefinery which recovered 81% of the main biomass fractions in a likely active form for the conversion of lipids and carbohydrates into biofuels and proteins into feed/food.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:18:33.816.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Araceli Bader
SpeciesList	scientific name: Aspergillus niger CBS 101883; NCBI TaxID: 1450533;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-30 03:09:13	ID requested
1	2023-03-10 15:42:59	announced
⏵ 2	2023-11-14 08:18:34	announced	2023-11-14: Updated project metadata.

Bader AN, Sanchez Rizza L, Consolo VF, Curatti L, Bioprospecting for fungal enzymes for applications in microalgal biomass biorefineries. Appl Microbiol Biotechnol, 107(2-3):591-607(2023) [pubmed]

submitter keyword: Aspergillus niger

biorefinery

glycoside hydrolases

microalga

secretomic analysis

Fabiana Consolo
contact affiliation	INBIOTEC
contact email	aracelibdr@gmail.com
lab head
Araceli Bader
contact affiliation	INBIOTEC-CONICET
contact email	aracelibdr@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034998
     1. Label: PRIDE project
     2. Name: bioprospecting for fungal enzymes for applications in microalgal-biomass based biorefineries