PXD034982

PXD034982 is an original dataset announced via ProteomeXchange.

Title	b1-integrin binding and lysosome targeting aptamer homes to triple-negative breast cancer in mice
Description	Targeted therapies for triple-negative breast cancer have increased the number of available treatment options for patients. However, an optimal treatment strategy is still an unmet medical need due to the lack of targetable biomarkers and tumour heterogeneity. Aptamers have high selectivity and specificity towards target proteins. Lower molecular weight, increased stability, less immunogenicity, and rapid tissue uptake make aptamers an attractive alternative to antibodies. Attempts to develop aptamer therapeutics have shown difficulties translating in vitro results to in vivo. Aptamer GreenB1 exhibits selectivity to triple-negative MDA-MB-231 human breast cancer cell line in vitro compared to estrogen, progesterone, and glucocorticoid receptor-expressing MCF-7 human breast cancer cell line. The aptamer is rapidly internalised into cells and trafficked to lysosomes. Here, we identify 1-integrin as the target protein for GreenB1 using proximity labelling and mass spectrometry proteomics. GreenB1 homes preferentially to the tumour in the 4T1 triple-negative breast cancer mice model in vivo.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:13:35.265.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD034982
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Karlis Pleiko
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-30 02:54:34	ID requested
1	2023-08-24 01:19:15	announced
⏵ 2	2023-11-14 07:13:35	announced	2023-11-14: Updated project metadata.

10.6019/PXD034982;

submitter keyword: b-integrin,aptamer, triple negative breast cancer

Una Riekstina
contact affiliation	Faculty of Medicine, Prof.
contact email	una.riekstina@lu.lv
lab head
Karlis Pleiko
contact affiliation	University of Latvia/University of Tartu
contact email	karlis.pleiko@lu.lv
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034982
     1. Label: PRIDE project
     2. Name: b1-integrin binding and lysosome targeting aptamer homes to triple-negative breast cancer in mice