PXD034978

PXD034978 is an original dataset announced via ProteomeXchange.

Title	Local burn wound environment verses systemic response: comparison of proteins and metabolites
Description	In this study, paired blood plasma (BP) and blister fluid (BF) samples from 5 paediatric burn patients were analysed using mass spectrometry to compare their protein and metabolite composition. The relative quantification of proteins was achieved through a label-free data independent acquisition mode. The relative quantification of metabolites was achieved using a Shimadzu Smart Metabolite Database gas chromatography mass spectrometry (GCMS) targeted assay. In total, 562 proteins and 141 individual metabolites were identified in the samples. There was 81% similarity in the proteins present in the BP and BF, with 50 and 54 unique proteins found in each sample type respectively. BF contained keratinocyte proliferation-related proteins and blood plasma contained abundant blood clotting proteins and apolipoproteins. BF contained more carbohydrates and less alpha-hydroxy acid metabolites than the BP. In this study, there were unique proteins and metabolites in BF and BP which were reflective of the local wound environment and systemic environments respectively. The results from this study demonstrate that the biomolecule content of BF is mostly the same as blood, but it also contains information specific to the local wound environment.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:56:43.663.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tuo Zang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600; Shimadzu instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-30 02:49:14	ID requested
1	2022-08-15 04:27:35	announced
⏵ 2	2023-11-14 08:56:44	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: burns, proteomics, child, wound fluid, metabolomics, plasma,paediatric

Leila Cuttle
contact affiliation	Queensland University of Technology (QUT), School of Biomedical Sciences, Faculty of Health, Centre for Children’s Health Research, South Brisbane, Queensland, Australia
contact email	leila.cuttle@qut.edu.au
lab head
Tuo Zang
contact affiliation	Queensland University of Technology
contact email	tuo.zang@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD034978

PRIDE project URI

• PRIDE
  1. PXD034978
     1. Label: PRIDE project
     2. Name: Local burn wound environment verses systemic response: comparison of proteins and metabolites