PXD034976 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Clickable glutathione-based Isotope Coded Affinity Label to quantify glutathionylation |
Description | Identification of cysteines with high oxidation susceptibility is important for understanding redox-mediated biological processes associated with health and disease. We developed a chemical proteomic strategy that helps find cysteines with high susceptibility to S-glutathionylation. Our strategy is based on the isotopically labelled clickable glutathione derivatives that can quantify relative levels of glutathionylated and reduced forms (SSG vs. SH) of cysteines in mass spectrometry, which is named 'Clickable Glutathione-based Isotope-coded Affinity-Tag (G-ICAT).' The G-ICAT approach was applied to the mouse C2C12 cell line upon addition of hydrogen peroxide, finding 1,518 glutathionylated cysteines and their relative levels of SSG over SH upon adding hydron peroxide. This chemical proteomic strategy will significantly contribute to identifying functional cysteines regulated by glutathionylation in redox signaling. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:32:29.001.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Young-Hoon Ahn |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | methylthiolated residue; acetylated residue; monohydroxylated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-06-30 02:48:20 | ID requested | |
1 | 2024-03-07 10:47:21 | announced | |
⏵ 2 | 2024-10-22 06:32:30 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1016/j.chembiol.2023.08.004; |
Kukulage DSK, Yapa Abeywardana M, Matarage Don NNJ, Hu RM, Shishikura K, Matthews ML, Ahn YH, Chemoproteomic strategy identified p120-catenin glutathionylation regulates E-cadherin degradation and cell migration. Cell Chem Biol, 30(12):1542-1556.e9(2023) [pubmed] |
Keyword List
submitter keyword: LC-MS/MS for glutathionylation in mouse C2C12 |
Contact List
Young-Hoon Ahn |
contact affiliation | Wayne State University, Department of Chemistry |
contact email | yahn@chem.wayne.edu |
lab head | |
Young-Hoon Ahn |
contact affiliation | Drexel University |
contact email | yahn@chem.wayne.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034976
- Label: PRIDE project
- Name: Clickable glutathione-based Isotope Coded Affinity Label to quantify glutathionylation