PXD034976

PXD034976 is an original dataset announced via ProteomeXchange.

Title	Clickable glutathione-based Isotope Coded Affinity Label to quantify glutathionylation
Description	Identification of cysteines with high oxidation susceptibility is important for understanding redox-mediated biological processes associated with health and disease. We developed a chemical proteomic strategy that helps find cysteines with high susceptibility to S-glutathionylation. Our strategy is based on the isotopically labelled clickable glutathione derivatives that can quantify relative levels of glutathionylated and reduced forms (SSG vs. SH) of cysteines in mass spectrometry, which is named 'Clickable Glutathione-based Isotope-coded Affinity-Tag (G-ICAT).' The G-ICAT approach was applied to the mouse C2C12 cell line upon addition of hydrogen peroxide, finding 1,518 glutathionylated cysteines and their relative levels of SSG over SH upon adding hydron peroxide. This chemical proteomic strategy will significantly contribute to identifying functional cysteines regulated by glutathionylation in redox signaling.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:32:29.001.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Young-Hoon Ahn
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	methylthiolated residue; acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-30 02:48:20	ID requested
1	2024-03-07 10:47:21	announced
⏵ 2	2024-10-22 06:32:30	announced	2024-10-22: Updated project metadata.

10.1016/j.chembiol.2023.08.004;

Kukulage DSK, Yapa Abeywardana M, Matarage Don NNJ, Hu RM, Shishikura K, Matthews ML, Ahn YH, Chemoproteomic strategy identified p120-catenin glutathionylation regulates E-cadherin degradation and cell migration. Cell Chem Biol, 30(12):1542-1556.e9(2023) [pubmed]

submitter keyword: LC-MS/MS for glutathionylation in mouse C2C12

Young-Hoon Ahn
contact affiliation	Wayne State University, Department of Chemistry
contact email	yahn@chem.wayne.edu
lab head
Young-Hoon Ahn
contact affiliation	Drexel University
contact email	yahn@chem.wayne.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD034976

PRIDE project URI

• PRIDE
  1. PXD034976
     1. Label: PRIDE project
     2. Name: Clickable glutathione-based Isotope Coded Affinity Label to quantify glutathionylation