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PXD034976

PXD034976 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleClickable glutathione-based Isotope Coded Affinity Label to quantify glutathionylation
DescriptionIdentification of cysteines with high oxidation susceptibility is important for understanding redox-mediated biological processes associated with health and disease. We developed a chemical proteomic strategy that helps find cysteines with high susceptibility to S-glutathionylation. Our strategy is based on the isotopically labelled clickable glutathione derivatives that can quantify relative levels of glutathionylated and reduced forms (SSG vs. SH) of cysteines in mass spectrometry, which is named 'Clickable Glutathione-based Isotope-coded Affinity-Tag (G-ICAT).' The G-ICAT approach was applied to the mouse C2C12 cell line upon addition of hydrogen peroxide, finding 1,518 glutathionylated cysteines and their relative levels of SSG over SH upon adding hydron peroxide. This chemical proteomic strategy will significantly contribute to identifying functional cysteines regulated by glutathionylation in redox signaling.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:32:29.001.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYoung-Hoon Ahn
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmethylthiolated residue; acetylated residue; monohydroxylated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-30 02:48:20ID requested
12024-03-07 10:47:21announced
22024-10-22 06:32:30announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.chembiol.2023.08.004;
Kukulage DSK, Yapa Abeywardana M, Matarage Don NNJ, Hu RM, Shishikura K, Matthews ML, Ahn YH, Chemoproteomic strategy identified p120-catenin glutathionylation regulates E-cadherin degradation and cell migration. Cell Chem Biol, 30(12):1542-1556.e9(2023) [pubmed]
Keyword List
submitter keyword: LC-MS/MS for glutathionylation in mouse C2C12
Contact List
Young-Hoon Ahn
contact affiliationWayne State University, Department of Chemistry
contact emailyahn@chem.wayne.edu
lab head
Young-Hoon Ahn
contact affiliationDrexel University
contact emailyahn@chem.wayne.edu
dataset submitter
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Dataset FTP location
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