PXD034942

PXD034942 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Bioinertization of nanoLC/MS/MS systems by depleting metal ions from the mobile phases for phosphoproteomics
Description	We have successfully bioinertized a nanoLC/MS/MS system for the highly sensitive analysis of phosphopeptides by depleting metal ions from the mobile phase. We found that not only the direct contact of phosphopeptides with metal materials, but also the indirect contact with HPLC pump through the mobile phase causes the significant loss in the recovery of phosphopeptides. Moreover, we observed that electrospray ionization was affected by the mobile phase containing multiple metal ions as well as the sample solutions contaminated with metal ions used by immobilized metal ion affinity chromatography for the phosphopeptide enrichment. To solve these problems, metal ions were depleted by inserting an on-line filter device containing metal chelating resins between the gradient mixer and the autosampler. As a result, we were able to increase the identification number of phosphopeptides by a factor of 1.5. This strategy would be applicable to highly sensitive analysis of other phosphorylated biomolecules by nanoLC/MS/MS.
HostingRepository	jPOST
AnnounceDate	2023-03-24
AnnouncementXML	Submission_2023-03-24_06:22:07.620.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD034942
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Komori Yumi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-27 04:38:37	ID requested
⏵ 1	2023-03-24 06:22:08	announced

Publication List

Komori Y, Niinae T, Imami K, Yanagibayashi J, Yasunaga K, Imamura S, Tomita M, Ishihama Y, Bioinertization of NanoLC/MS/MS Systems by Depleting Metal Ions From the Mobile Phases for Phosphoproteomics. Mol Cell Proteomics, 22(5):100535(2023) [pubmed]

Komori Y, Niinae T, Imami K, Yanagibayashi J, Yasunaga K, Imamura S, Tomita M, Ishihama Y, Bioinertization of NanoLC/MS/MS Systems by Depleting Metal Ions From the Mobile Phases for Phosphoproteomics. Mol Cell Proteomics, 22(5):100535(2023) [pubmed]

Keyword List

submitter keyword: bioinert, phosphopeptides, nanoLC/MS/MS

submitter keyword: bioinert, phosphopeptides, nanoLC/MS/MS

Contact List

Yasushi Ishihama
lab head
Komori Yumi
contact affiliation	Kyoto University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001671/