PXD034941

PXD034941 is an original dataset announced via ProteomeXchange.

Title	RNA-host protein interaction analyses reveal additional proteins involved in SARS-CoV-2 RNA biology
Description	SARS-CoV-2 is a positive single-stranded RNA virus which interacts at different stages with the host proteins of infected cells. These interactions are necessary for the host to recognize and block the replication of the virus. But, at the same time, the virus requires host proteins to translate, transcribe and replicate its genetic material. In order to identify the host proteins that interact with SARS-CoV-2 RNA, we adopted the RNA-protein interaction detection coupled to mass spectrometry (RaPID-MS) technology, which allows the purification and identification by MS-based proteomics of the proteins associated to a specific RNA of interest expressed in mammalian cells. In particular, we conducted the analysis on the more structured regions of SARS-CoV-2 RNA and retrieved several proteins specifically associated with each region. Overall, our data revealed a list of proteins associated to SARS-CoV-2 RNA that will be further characterized to understand their role in SARS-CoV-2 infection and viral replication.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_12:48:12.018.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roberto Giambruno
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-27 02:53:45	ID requested
⏵ 1	2023-11-14 12:48:12	announced

Dataset with its publication pending

ProteomeXchange project tag: Sars-cov-2, Covid-19

submitter keyword: ARS-CoV-2

interactome

host-virus interactions

RBPs

Francesco Nicassio
contact affiliation	Italian Institute of Technology
contact email	francesco.nicassio@iit.it
lab head
Roberto Giambruno
contact affiliation	ITB-CNR
contact email	roberto.giambruno@itb.cnr.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD034941

PRIDE project URI

• PRIDE
  1. PXD034941
     1. Label: PRIDE project
     2. Name: RNA-host protein interaction analyses reveal additional proteins involved in SARS-CoV-2 RNA biology