PXD034924

PXD034924 is an original dataset announced via ProteomeXchange.

Title	Histone modifications associated with the histone chaperone DAXX
Description	A multitude of histone chaperones is required to protect histones from their biosynthesis to DNA deposition. They cooperate through the formation of co-chaperone complexes, but the crosstalk between nucleosome assembly pathways is unclear. Using explorative interactomics approaches, we map the organization of the histone H3-H4 chaperones network and define the interplay between histone chaperones systems. We identify and validate a panel of novel histone (PTM) dependent complexes. We show DAXX acts separately from the rest of the network, recruiting heterochromatin factors and promoting lysine 9 tri-methylated new histone H3.3 prior to deposition onto DNA. With its functionality, DAXX provides a molecular mechanism for de novo heterochromatin assembly.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:28:36.308.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Victor Solis
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monomethylated residue; acetylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-26 07:51:01	ID requested
1	2023-03-06 04:25:30	announced
⏵ 2	2023-11-14 07:28:37	announced	2023-11-14: Updated project metadata.

Carraro M, Hendriks IA, Hammond CM, Solis-Mezarino V, V, ö, lker-Albert M, Elsborg JD, Weisser MB, Spanos C, Montoya G, Rappsilber J, Imhof A, Nielsen ML, Groth A, DAXX adds a de novo H3.3K9me3 deposition pathway to the histone chaperone network. Mol Cell, 83(7):1075-1092.e9(2023) [pubmed]

submitter keyword: DAXX, PTMs, H3.3, Histones

Anja Groth
contact affiliation	Novo Nordisk Center for Protein Research (CPR), Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark. Biotech Research and Innovation Centre (BRIC), Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark.
contact email	anja.groth@cpr.ku.dk
lab head
Victor Solis
contact affiliation	EpiQMAx GmbH
contact email	solis@genzentrum.lmu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD034924

PRIDE project URI

• PRIDE
  1. PXD034924
     1. Label: PRIDE project
     2. Name: Histone modifications associated with the histone chaperone DAXX