PXD034917

PXD034917 is an original dataset announced via ProteomeXchange.

Title	Chaperonin CCT controls cell fuelling by lipids and extracellular vesicle production through kinesin dynamics
Description	Please provide an overall description of your study, think something similar in scope to the manuscript abstractCell proteostasiCell proteostasis includes gene transcription, protein translation, folding of de novo proteins, post-translational modification, secretion, degradation and recycling. By profiling the proteome of extracellular vesicles (EVs) from T cells, we have found the chaperonin complex CCT, involved in the correct folding of particular proteins. By limiting CCT cell-content by siRNA, cells undergo altered lipid composition and metabolic rewiring towards a lipid-dependent metabolism, with increased activity of peroxisomes and mitochondria. This is due to dysregulation of the dynamics of interorganellar contacts between lipid droplets, mitochondria, peroxisomes and the endolysosomal system. This process accelerates the biogenesis of multivesicular bodies leading to higher EV production through the dynamic regulation of microtubule-based kinesin motors. These findings connect proteostasis with lipid metabolism through an unexpected role of CCT.s includes gene transcription, protein translation, folding of de novo proteins, post-translational modification, secretion, degradation and recycling. By profiling the proteome of extracellular vesicles (EVs) from T cells, we have found the chaperonin complex CCT, involved in the correct folding of particular proteins. By limiting CCT cell-content by siRNA, cells undergo altered lipid composition and metabolic rewiring towards a lipid-dependent metabolism, with increased activity of peroxisomes and mitochondria. This is due to dysregulation of the dynamics of interorganellar contacts between lipid droplets, mitochondria, peroxisomes and the endolysosomal system. This process accelerates the biogenesis of multivesicular bodies leading to higher EV production through the dynamic regulation of microtubule-based kinesin motors. These findings connect proteostasis with lipid metabolism through an unexpected role of CCT.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:54:43.109.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Enrique Calvo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-26 07:46:43	ID requested
1	2023-07-20 10:45:40	announced
2	2023-11-14 09:02:38	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:54:44	announced	2024-10-22: Updated project metadata.

Rojas-G, ó, mez A, Dosil SG, Chich, ó, n FJ, Fern, á, ndez-Gallego N, Ferrarini A, Calvo E, Calzada-Fraile D, Requena S, Ot, ó, n J, Serrano A, Tarifa R, Arroyo M, Sorrentino A, Pereiro E, V, á, zquez J, Valpuesta JM, S, á, nchez-Madrid F, Mart, í, n-C, ó, freces NB, Chaperonin CCT controls extracellular vesicle production and cell metabolism through kinesin dynamics. J Extracell Vesicles, 12(6):e12333(2023) [pubmed]

10.1002/jev2.12333;

submitter keyword: extracellular vesicle, lipidomic, peroxisome,CCT, lipid droplet, chaperonin

Enrique Calvo
contact affiliation	CNIC
contact email	ecalvo@cnic.es
lab head
Enrique Calvo
contact affiliation	CNIC
contact email	ecalvo@cnic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD034917

PRIDE project URI

• PRIDE
  1. PXD034917
     1. Label: PRIDE project
     2. Name: Chaperonin CCT controls cell fuelling by lipids and extracellular vesicle production through kinesin dynamics