PXD034916

PXD034916 is an original dataset announced via ProteomeXchange.

Title	Systemic knockout of the guanine-rich RNA sequence binding factor 1 gene (Grsf1-/-) induces gender specific developmental retardation in male individuals when aging.
Description	The guanine-rich RNA sequence binding factor 1 (GRSF1) is an RNA-binding protein of the heterogenous nuclear ribonucleoprotein H/F (hnRNP H/F) family that binds to guanine-rich RNA sequences forming G-quadruplex structures. In mice and humans there are single copy GRSF1 genes, but multiple transcripts have been reported. The RNA-binding protein GRSF1 has been implicated in a number of physiological processes (e.g. embryogenesis, erythropoiesis, redox homeostasis, RNA metabolism) but also in the pathogenesis of viral infections and hyperproliferative diseases. These postulated biological functions of GRSF1 originates from in vitro studies rather than complex in vivo systems. To assess the in vivo relevance of these findings, we created systemic Grsf1-/- knockout mice lacking exons 4 and 5 of the Grsf1 gene and compared the basic functional characteristics of these animals with those of wildtype controls. We found that Grsf1-deficient mice are viable, reproduce normally and have fully functional hematopoietic systems. Up to an age of 15 weeks they develop normally but when male individuals grow older, they gain significantly less body weight than wildtype controls in a gender specific way. Profiling Grsf1 mRNA expression in different mouse tissues we observed high concentrations in testis. Comparison of the testicular transcriptomes of Grsf1-/- mice and wildtype controls confirmed near complete knock-out of Grsf1 but otherwise subtle differences in transcript regulations. Comparative testicular proteome analyses suggested perturbed mitochondrial respiration in Grsf1-/- mice which may be related to compromised expression of complex I proteins. Here we present, for the first time, an in vivo complete Grsf1 knock-out mouse with comprehensive physiological, transcriptomic and proteomic characterization to improve our understanding of the GRSF1 beyond in vitro cell culture models.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:28:08.180.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Moritz Lassé
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-26 07:46:26	ID requested
1	2023-01-05 03:01:08	announced
⏵ 2	2023-11-14 08:28:10	announced	2023-11-14: Updated project metadata.

Dumoulin B, Heydeck D, J, ä, hn D, Lass, é M, Sofi S, Ufer C, Kuhn H, ) gain less body weight during adolescence and adulthood. Cell Biosci, 12(1):199(2022) [pubmed]

submitter keyword: gene expression regulation, lipoxygenase, embryogenesis, hematopoiesis, RNA binding proteins, redox homeostasis

Markus M. Rinschen
contact affiliation	III. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
contact email	m.rinschen@uke.de
lab head
Moritz Lassé
contact affiliation	Zentrum für Innere Medizin, III. Medizinische Klinik und Poliklinik (Nephrologie/Rheumatologie/Endokrinologie), Universitätsklinikum Hamburg-Eppendorf (UKE), Hamburg, Germany
contact email	moritz.lasse@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034916
     1. Label: PRIDE project
     2. Name: Systemic knockout of the guanine-rich RNA sequence binding factor 1 gene (Grsf1-/-) induces gender specific developmental retardation in male individuals when aging.