PXD034913 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of a targeted functional genomic screen in human skeletal muscle organoids |
Description | To begin to validate potential causal regulators of muscle function, we targeted genes containing novel skeletal muscle pQTLs and molecular/phenotypic associations, and performed a functional genomic screen in human skeletal muscle organoids (PMID: 30527761). We focused on proteins with negative associations to lean mass, grip strength or other metabolic associations, and generated a total of 27 individual rAAV6:shRNAs. Organoids were grown around contraction posts to monitor contractile force production during electrical stimulation, and transduced following differentiation and maturation to limit effects on the myogenic program (Figure 3A). Electrical stimulation was performed to induce either a tetanic contraction for assessment of maximum force production or stimulated with sustained lower frequency for assessment of fatigue. Following the protocol, organoids were analysed by proteomics which quantified 17/27 targets with 13 targets significantly reduced in abundance by rAAV6:shRNA. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_07:38:37.562.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Benjamin Parker |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-06-26 07:43:44 | ID requested | |
1 | 2023-01-08 15:41:50 | announced | |
⏵ 2 | 2023-11-14 07:38:39 | announced | 2023-11-14: Updated project metadata. |
Publication List
Molendijk J, Blazev R, Mills RJ, Ng YK, Watt KI, Chau D, Gregorevic P, Crouch PJ, Hilton JBW, Lisowski L, Zhang P, Reue K, Lusis AJ, Hudson JE, James DE, Seldin MM, Parker BL, Proteome-wide systems genetics identifies UFMylation as a regulator of skeletal muscle function. Elife, 11():(2022) [pubmed] |
Keyword List
submitter keyword: organoids, proteomics,muscle |
Contact List
Benjamin Parker |
contact affiliation | The University of Melbourne |
contact email | ben.parker@unimelb.edu.au |
lab head | |
Benjamin Parker |
contact affiliation | The University of Melbourne |
contact email | ben.parker@unimelb.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034913
- Label: PRIDE project
- Name: Proteomic analysis of a targeted functional genomic screen in human skeletal muscle organoids