PXD034898

PXD034898 is an original dataset announced via ProteomeXchange.

Title	Comparative surfaceome analysis of clonal Histomonas meleagridis strains with different pathogenicity reveals strain-dependent profiles
Description	Surface proteome of microbial pathogens is a crucial component of host-pathogen interactions. We applied cell surface biotinylation in association with high-throughput proteomic analysis to identify and quantify the surface proteome of a virulent and an attenuated strain, originating from the same single cell of H. meleagridis. In total 1485 proteins were identified amongst the two strains. Quantification revealed 22 and 45 up-regulated proteins in the virulent and in the attenuated strain, respectively. In the virulent strain proteins such as alpha-amylase, Clan CD family C13 asparaginyl endopeptidase-like cysteine peptidase, two Clan SC family S33, methylesterase-like serine peptidases, LysM peptidoglycan-binding domain-containing protein and surfactant B were up-regulated and could be linked to putative virulence factors involved in the colonization of the host and establishment of an infection. In the attenuated strain structural proteins such as actin-related proteins, fimbrin, coronin, were up-regulated, alongside with various transport and energy production proteins. Our results speak for the protozoan’s adaptation to the in vitro environment, with a clear tendency for up-regulation of mainly metabolic and structural proteins. We present a comprehensive profiling of H. meleagridis surface proteome. These results provide not only a better understanding of the surface molecules that may participate in establishing and maintaining infection but also highlights the pathogen’s in vitro adaptation processes.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:17:52.673.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ivana BILIC
SpeciesList	scientific name: Histomonas meleagridis; NCBI TaxID: 135588;
ModificationList	biotinylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-26 07:30:24	ID requested
1	2023-03-10 13:29:47	announced
⏵ 2	2023-11-14 08:17:53	announced	2023-11-14: Updated project metadata.

Ramires MJ, Hummel K, Hatfaludi T, Riedl P, Hess M, Bilic I, Strains with Different Pathogenicity Reveals Strain-Dependent Profiles. Microorganisms, 10(10):(2022) [pubmed]

submitter keyword: attenuation,Surface proteome, protozoa, virulence factors, Histomonas meleagridis, comparative proteomic analysis, LC-MS/MS

Ivana Bilic
contact affiliation	University Clinic for Poultry and Fish Medicine, University of Veterinary Medicine, Vienna, Austria
contact email	ivana.bilic@vetmeduni.ac.at
lab head
Ivana BILIC
contact affiliation	Clinic for Poultry and Fish Medicine, University of Veterinary Medicine, Vienna
contact email	ivana.bilic@vetmeduni.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034898
     1. Label: PRIDE project
     2. Name: Comparative surfaceome analysis of clonal Histomonas meleagridis strains with different pathogenicity reveals strain-dependent profiles