PXD034880

PXD034880 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Regulation of urea cycle by reversible high stoichiometry lysine succinylation
Description	The posttranslational modification, lysine succinylation is implicated in the regulation of various metabolic pathways. However, its biological relevance remains uncertain due to methodological difficulties in determining high impact succinylation sites. In the present study, using stable isotope labeling and data independent acquisition mass spectrometry, we quantified lysine succinylation stoichiometries in mouse livers. Despite the low overall stoichiometry of lysine succinylation, several high stoichiometry sites were identified, especially upon deletion of the desuccinylase SIRT5. In particular, multiple high stoichiometry lysine sites identified in argininosuccinate synthase ASS1, a key enzyme in urea cycle, are regulated by SIRT5. Mutation of the high stoichiometry lysine in ASS1 to succinyl mimetic glutamic acid significantly decreased its enzymatic activity. Metabolomics profiling confirms that SIRT5 deficiency decreases urea cycle activity in liver. Importantly, SIRT5 deficiency compromises ammonia tolerance and reduces locomotor and exploratory activity in male mice upon high ammonium diet feeding. Therefore, lysine succinylation is functionally important in ammonia metabolism.
HostingRepository	MassIVE
AnnounceDate	2023-12-08
AnnouncementXML	Submission_2023-12-08_15:34:21.209.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Birgit Schilling
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	N6-succinyl-L-lysine
Instrument	TripleTOF 6600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-24 15:53:27	ID requested
⏵ 1	2023-12-08 15:34:21	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: data independent acquisition, sirtuin

submitter keyword: data independent acquisition, sirtuin

Contact List

Birgit Schilling
contact affiliation	Buck Institute
contact email	bschilling@buckinstitute.org
lab head
Birgit Schilling
contact affiliation	Buck Institute
contact email	bschilling@buckinstitute.org
dataset submitter

Full Dataset Link List

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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000089726/