PXD034878

PXD034878 is an original dataset announced via ProteomeXchange.

Title	A novel overlapping gene azyx-1 affects the translation of zyxin in C. elegans
Description	Overlapping genes are widely prevalent, however, their expression and consequences are poorly understood. Here, we describe and functionally characterize a novel zyx-1 overlapping gene, azyx-1, with distinct regulatory functions in C. elegans. We observed conservation of alternative open reading frames overlapping the 5’ region of zyxin family members in several animal species, and find shared sites of azyx-1 and zyxin proteoform expression in C. elegans. In line with a standard ribosome scanning model, our results support cis regulation of zyx-1 long isoform(s) by upstream initiating azyx-1a. Moreover, we report on a rare observation of trans regulation of zyx-1 by azyx-1, with evidence of increased ZYX-1 upon azyx-1 overexpression. Our results suggest a dual role for azyx-1 in influencing zyx-1 proteoform heterogeneity and highlights its impact on C. elegans muscular integrity and locomotion.
HostingRepository	PanoramaPublic
AnnounceDate	2023-07-19
AnnouncementXML	Submission_2023-07-19_08:30:22.947.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christina Ludwig
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	Acetyl; Carbamidomethyl
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-24 13:33:09	ID requested
⏵ 1	2023-07-19 08:30:23	announced

Dataset with its publication pending

submitter keyword: overlapping gene, non-canonical, PRM, targeted proteomics

Christina Ludwig
contact affiliation	Bavarian Center for Biomolecular Mass Spectrometry (BayBioMS), TU Munich
contact email	tina.ludwig@tum.de
lab head
Christina Ludwig
contact affiliation	Bavarian Center for Biomolecular Mass Spectrometry (BayBioMS), TU Munich
contact email	tina.ludwig@tum.de
dataset submitter

Panorama Public dataset URI