PXD034871

PXD034871 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Structure of the human heparan sulfate polymerase complex EXT1-EXT2
Description	Heparan sulfates are complex polysaccharides that mediate the interaction with a broad range of protein ligands at the cell surface. A key step in heparan sulfate biosynthesis is catalyzed by the bi-functional glycosyltransferases EXT1 and EXT2, which generate the glycan backbone consisting of repeating Nacetylglucosamine and glucuronic acid units. The molecular mechanism of heparan sulfate chain polymerization remains, however, unknown. Human EXT1 and EXT2, lacking the N-terminal transmembrane helix, were co-expressed as a secreted complex using human embryonic kidney cells (FreeStyle 293-F). We purified 0.5 mg of the complex from 900 mL suspension culture using two immobilized metal affinity chromatography steps and an interspersed protease treatment to cleave off the alkaline phosphatase fusion protein serving as a secretion signal. Mass spectrometry-based proteomic analysis confirmed the quality of the sample with EXT1 and EXT2 found to be the most abundant proteins, and being present in comparable amounts.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:18:30.154.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD034871
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-24 08:43:30	ID requested
1	2022-12-09 04:28:01	announced
⏵ 2	2023-11-14 07:18:31	announced	2023-11-14: Updated project metadata.

Publication List

10.6019/PXD034871;
Leisico F, Omeiri J, Le Narvor C, Beaudouin J, Hons M, Fenel D, Schoehn G, Cout, é Y, Bonnaff, é D, Sadir R, Lortat-Jacob H, Wild R, Structure of the human heparan sulfate polymerase complex EXT1-EXT2. Nat Commun, 13(1):7110(2022) [pubmed]

10.6019/PXD034871;

Leisico F, Omeiri J, Le Narvor C, Beaudouin J, Hons M, Fenel D, Schoehn G, Cout, é Y, Bonnaff, é D, Sadir R, Lortat-Jacob H, Wild R, Structure of the human heparan sulfate polymerase complex EXT1-EXT2. Nat Commun, 13(1):7110(2022) [pubmed]

Keyword List

submitter keyword: EXT2, EXT1,Cell surface, nanoLC-MS/MS, heparan sulfates

submitter keyword: EXT2, EXT1,Cell surface, nanoLC-MS/MS, heparan sulfates

Contact List

Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
lab head
Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD034871

PRIDE project URI

Repository Record List

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