PXD034863

PXD034863 is an original dataset announced via ProteomeXchange.

Title	Outer membrane utilisomes mediate oligosaccharide uptake in gut Bacteroidetes
Description	Bacteroidetes are abundant members of the human microbiota, and species occupying the distal gut are capable of utilising a myriad of diet- and host-derived glycans. Transport of glycans across the outer membrane (OM) of these bacteria is mediated by SusCD protein complexes. Additionally, cell surface-exposed lipoproteins, namely surface glycan binding proteins and glycoside hydrolases, play critical roles in the capture and processing of large glycan chains into transport-competent substrates. Here we show that, for the levan and dextran utilisation systems of Bacteroides thetaiotaomicron, the additional OM components assemble on the core SusCD transporter, forming stable glycan utilising machines which we term ‘utilisomes’. Single particle electron cryogenic electron microscopy (cryo-EM) structures in the absence and presence of substrate reveal concerted conformational changes that rationalise the role of each component for efficient nutrient capture, as well as providing a direct demonstration of the pedal bin mechanism of substrate capture in the intact utilisome.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:48:58.138.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthias Trost
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Bacteroides thetaiotaomicron; NCBI TaxID: 818;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-24 08:30:21	ID requested
1	2023-06-19 06:48:43	announced
2	2023-11-14 09:01:29	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:48:58	announced	2024-10-22: Updated project metadata.

10.1038/s41586-023-06146-w;

White JBR, Silale A, Feasey M, Heunis T, Zhu Y, Zheng H, Gajbhiye A, Firbank S, Basl, é A, Trost M, Bolam DN, van den Berg B, Ranson NA, Outer membrane utilisomes mediate glycan uptake in gut Bacteroidetes. Nature, 618(7965):583-589(2023) [pubmed]

submitter keyword: Outer membrane proteome,Microbiota, Glycan utilisation, Gut Bacteroidetes

Matthias Trost
contact affiliation	Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom
contact email	matthias.trost@ncl.ac.uk
lab head
Matthias Trost
contact affiliation	Newcastle University
contact email	matthias.trost@ncl.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD034863

PRIDE project URI

• PRIDE
  1. PXD034863
     1. Label: PRIDE project
     2. Name: Outer membrane utilisomes mediate oligosaccharide uptake in gut Bacteroidetes