PXD034853 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of rabbit fresh and cryopreserved semen (seminal plasma) |
Description | Cryoinjury and protein changes are a consequence of cryopreservation and may have a negative impact on sperm quality regarding motility, viability and fertilizing ability. However, potential proteomic changes in rabbit semen throughout the cryopreservation process have never been investigated previously. The aim of the present study was to compare the whole proteome of fresh and cryopreserved rabbit extracellular fluid of semen. Comparative analysis and identification of proteins were performed using 2-dimensional difference in-gel electrophoresis (2D-DIGE) coupled with matrix-assisted laser desorption/ionization mass (MALDI TOF/TOF) spectrometry. We found that 28 proteins differed in abundance between fresh and frozen extracellular fluid. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_06:51:27.418.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mariola Słowińska |
SpeciesList | scientific name: Oryctolagus cuniculus (Rabbit); NCBI TaxID: 9986; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | autoflex |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-06-22 15:09:01 | ID requested | |
1 | 2022-10-13 02:46:31 | announced | |
⏵ 2 | 2023-11-14 06:51:28 | announced | 2023-11-14: Updated project metadata. |
Publication List
Rusco G, S, ł, owi, ń, ska M, Di Iorio M, Cerolini S, Maffione AB, Ciereszko A, Iaffaldano N, Proteomic analysis of rabbit fresh and cryopreserved semen provides an important insight into molecular mechanisms of cryoinjuries to spermatozoa. Theriogenology, 191():77-95(2022) [pubmed] |
Keyword List
submitter keyword: seminal plasma,rabbit, MALDI TOF/TOF |
Contact List
Mariola Słowińska |
contact affiliation | Institute of Animal Reproduction and Food Research of Polish Academy of Sciences |
contact email | m.slowinska@pan.olsztyn.pl |
lab head | |
Mariola Słowińska |
contact affiliation | Institute of Animal Reproduction and Food Research of Polish Academy of Sciences |
contact email | m.slowinska@pan.olsztyn.pl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD034853 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034853
- Label: PRIDE project
- Name: Proteomic analysis of rabbit fresh and cryopreserved semen (seminal plasma)