PXD034844

PXD034844 is an original dataset announced via ProteomeXchange.

Title	Reanalysis of PXD009319: "An alliance of gel-based and gel-free proteomic techniques displays substantial insight into the proteome of a virulent and an attenuated Histomonas meleagridis strain"
Description	The current work aimed to detect and identify significantly differentially expressed proteins between a virulent and an attenuated Histomonas meleagridis strain which were in vitro co-cultivated with Escherichia coli DH5alpha. This was done by applying 2D-DIGE and sequential window acquisition of all theoretical spectra (SWATH) MS as tools on the two well-defined strains. In the gel-based experiments, 49 identified protein spots were found to be differentially expressed, of which 37 belonged to the in vitro cultivated virulent strain and 12 to the attenuated one. The most frequently identified proteins in the virulent strain take part in cytoskeleton formation, carbohydrate metabolism and adaptation to stress. However, post-translationally modified or truncated ubiquitous cellular proteins such as actin and GAPDH were identified as upregulated in multiple gel positions. This indicated their contribution to processes not related to cytoskeleton and carbohydrate metabolism, such as fibronectin or plasminogen binding. On the other hand, proteins involved in cytoskeleton, cell division and cytoskeleton organization were frequently observed in the attenuated strain. The findings of the gel-based studies were supplemented by the gel-free SWATH MS analysis, which identified and quantified 42 significantly differentially regulated proteins. In this case proteins with peptidase activity, metabolic proteins and actin-regulating proteins were the most frequent findings in the virulent strain, while proteins involved in hydrogenosomal carbohydrate metabolism dominated the results in the attenuated one.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:36:44.978.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ivana BILIC
SpeciesList	scientific name: Histomonas meleagridis; NCBI TaxID: 135588;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-22 14:51:42	ID requested
1	2023-03-10 20:17:30	announced
⏵ 2	2023-11-14 08:36:47	announced	2023-11-14: Updated project metadata.

Ramires MJ, Hummel K, Hatfaludi T, Riedl P, Hess M, Bilic I, Strains with Different Pathogenicity Reveals Strain-Dependent Profiles. Microorganisms, 10(10):(2022) [pubmed]

submitter keyword: attenuation, protozoa, SWATH MS,Histomonas meleagridis, virulence factors, comparative proteomic analysis, LC-MS/MS

Ivana Bilic
contact affiliation	University Clinic for Poultry and Fish Medicine, University of Veterinary Medicine, Vienna, Austria
contact email	Ivana.bilic@vetmeduni.ac.at
lab head
Ivana BILIC
contact affiliation	Clinic for Poultry and Fish Medicine, University of Veterinary Medicine, Vienna
contact email	ivana.bilic@vetmeduni.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD034844

PRIDE project URI

• PRIDE
  1. PXD034844
     1. Label: PRIDE project
     2. Name: Reanalysis of PXD009319: "An alliance of gel-based and gel-free proteomic techniques displays substantial insight into the proteome of a virulent and an attenuated Histomonas meleagridis strain"