PXD034842

PXD034842 is an original dataset announced via ProteomeXchange.

Title	ClpP protease modulates bacterial growth, stress response and bacterial virulence in Brucella abortus
Description	Intracellular proteolysis via ATP-dependent proteases is a conserved biological process. The ATP-dependent Clp protease is made up of peptidase ClpP and ATP-dependent chaperones and plays an important role in the proteolysis process. In several pathogenic bacteria, the Clp protease is implicated in the stress reponses and contributes to the bacterial virulence. In brucella abortus, the ΔclpP mutant strain exhibited a pronounced growth defect in TSB medium and the results showed that the ΔclpP mutant was sensitive to high temperature, high osmotic environment and iron deficient environment. In addition, deletion of the clpP significantly affected Brucella virulence in macrophage and mice infection models. Based on the iTRAQ analysis, the different expressed proteins were mainly involved in amino acid transport and metabolism, energy production and conversion, and secondary metabolites biosynthesis, transport and catabolism. Overall, our study revealed preliminary molecular mechanism between Clp protease, bacterial growth, stress response and bacterial virulence in Brucella strains
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:07:17.798.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	dongjie sun
SpeciesList	scientific name: Brucella abortus (strain 2308); NCBI TaxID: 359391;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-22 14:18:42	ID requested
1	2023-10-24 11:13:21	announced
⏵ 2	2023-11-14 09:07:26	announced	2023-11-14: Updated project metadata.

Sun D, Liu Y, Peng X, Dong H, Jiang H, Fan X, Feng Y, Sun J, Han K, Gao Q, Niu J, Ding J, ClpP protease modulates bacterial growth, stress response, and bacterial virulence in Brucella abortus. Vet Res, 54(1):68(2023) [pubmed]

submitter keyword: virulence, iTRAQ analysis,Brucella, ClpP protease

Jiabo Ding
contact affiliation	Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China
contact email	dingjiabo@126.com
lab head
dongjie sun
contact affiliation	Institute of Animal Sciences, Chinese Academy of Agricultural Sciences
contact email	82101211278@caas.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034842
     1. Label: PRIDE project
     2. Name: ClpP protease modulates bacterial growth, stress response and bacterial virulence in Brucella abortus