PXD034838
PXD034838 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic analysis of prostate cancer FFPE samples reveals markers of disease progression and aggressiveness |
| Description | Prostate cancer (PCa) is the second most common cancer in men. Diagnosis and risk assessment are widely based on serum Prostate Specific Antigen (PSA) and biopsy which have low accuracy. Towards the discovery of biomarkers for better patient stratification, we performed proteomic analysis of Formalin Fixed Paraffin Embedded (FFPE) prostate tissue specimens following optimized sample preparation protocol and LC-MS/MS. Comparative analysis of 86 PCa samples among grade groups 1-5 identified 301 differentially expressed proteins involved in metabolism, angiogenesis, response to stress and cytoskeleton organization. Additional analysis based on biochemical recurrence (BCR; BCR+ n=14, BCR- n=51) revealed 197 differentially expressed proteins that indicate disease persistence. Filtering the overlapping proteins of these analyses, seven proteins (NPM1, UQCRH, HSPA9, MRPL3, VCAN, SERBP1, HSPE1) had increased expression in advanced grades and in BCR+/BCR- and may play a critical role in PCa aggressiveness and persistence. Notably, our observations for NPM1, UQCRH and VCAN were validated in The Cancer Genome Atlas (TCGA), where they were upregulated in BCR+/BCR-. UQCRH levels were also associated with 5-year survival. Our study provides valuable insights on the key regulators of PCa progression and aggressiveness. The seven selected proteins could be used for the development of risk assessment tools. |
| HostingRepository | MassIVE |
| AnnounceDate | 2022-08-01 |
| AnnouncementXML | Submission_2022-08-01_12:19:59.600.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Vasiliki |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | iodoacetamide - site C; S-carboxamidomethyl-L-cysteine |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-06-22 08:49:02 | ID requested | |
| ⏵ 1 | 2022-08-01 12:19:59 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Prostate cancer, proteomics, biochemical recurrence, biomarkers, FFPE samples, geLC-MS/MS |
Contact List
| Antonia Vlahou | |
|---|---|
| contact affiliation | Biomedical Research Foundation, Academy of Athens |
| contact email | vlahoua@bioacademy.gr |
| lab head | |
| Vasiliki | |
| contact affiliation | Biomedical Research Foundation, Academy of Athens |
| contact email | vlygirou@bioacademy.gr |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000089707/ |
to receive all new ProteomeXchange dataset release announcements!
