PXD034837
PXD034837 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | LFQ of immunoprecipitates with GFP-Trap from GFP-, OfMasc-GFP-, or Del1-OfMasc-GFP-transfected cells |
| Description | OfMasc-GFP derivatives or GFP were transiently expressed in OfTC cells. At 2 days post transfection, OfTC cells were treated with 100 nM Bortezomib for 1 day. The OfT1C cells were then fixed with 0.1% formaldehyde, and the fixation was quenched with 300 mM glycine. The cells were lysed on ice in 1 mL of RIPA buffer. After centrifugation, the supernatants were incubated with GFP-Trap Magnetic Agarose (ChromoTek) for 3 h at 4ºC. Proteins bound to the beads were digested by adding 200 ng of Trypsin/Lys-C mix (Promega) for 16 h at 37ºC. The digests were reduced, alkylated, acidified with trifluoroacetic acid, and desalted using a GL-Tip SDB (GL Sciences). LC-MS/MS analysis of the resultant peptides was performed using an EASY-nLC 1200 UHPLC connected to an Orbitrap Fusion mass spectrometer equipped with a nanoelectrospray ion source (Thermo Fisher Scientific). Raw data were directly analyzed against the wFur encoded protein data predicted from an in-house assembled wFur draft genomic sequence and O. furnacalis protein data ‘GCF_004193835.1_ASM419383v1_protein.faa’ downloaded from NCBI supplemented with OfMasc-GFP and GFP-Trap sequences using Proteome Discoverer version 2.4 (Thermo Fisher Scientific) with Sequest HT search engine. Label-free precursor ion quantification was performed using the precursor ions quantifier node. |
| HostingRepository | jPOST |
| AnnounceDate | 2022-10-04 |
| AnnouncementXML | Submission_2022-11-15_09:31:39.922.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Hidetaka Kosako |
| SpeciesList | scientific name: cellular organisms; NCBI TaxID: 131567; |
| ModificationList | L-methionine sulfoxide; alpha-amino acetylated residue; S-carboxamidomethyl-L-cysteine |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-06-22 03:20:05 | ID requested | |
| 1 | 2022-10-04 01:46:19 | announced | |
| ⏵ 2 | 2022-11-15 09:31:40 | announced | 2022-11-16: Updated PubMed. |
Keyword List
| submitter keyword: GFP-Trap, OfMasc |
Contact List
| Hidetaka Kosako | |
|---|---|
| lab head | |
| Hidetaka Kosako | |
| contact affiliation | Tokushima University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001673/ |
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