PXD034832

PXD034832 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of rabbit fresh and cryopreserved semen (spermatozoa)
Description	Cryoinjury and protein changes are a consequence of cryopreservation and may have a negative impact on sperm quality regarding motility, viability and fertilizing ability. However, potential proteomic changes in rabbit semen throughout the cryopreservation process have never been investigated previously. The aim of the present study was to compare the whole proteome of fresh and cryopreserved rabbit spermatozoa. Comparative analysis and identification of proteins were performed using 2-dimensional difference in-gel electrophoresis (2D-DIGE) coupled with matrix-assisted laser desorption/ionization mass (MALDI TOF/TOF) spectrometry. We found that 108 proteins differed in abundance in spermatozoa between fresh and frozen semen. Most of these proteins was involved in pathways related to energy metabolism and protein quality control under stress conditions, reproductive processes and mechanisms of cell death/survival regulation, resulting in a significant decrease of motility and viability of post-thawing rabbit sperm and its potential fertilizing ability.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:24:40.901.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mariola Słowińska
SpeciesList	scientific name: Oryctolagus cuniculus (Rabbit); NCBI TaxID: 9986;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-22 02:33:57	ID requested
1	2022-10-13 23:07:35	announced
⏵ 2	2023-11-14 08:24:42	announced	2023-11-14: Updated project metadata.

Rusco G, S, ł, owi, ń, ska M, Di Iorio M, Cerolini S, Maffione AB, Ciereszko A, Iaffaldano N, Proteomic analysis of rabbit fresh and cryopreserved semen provides an important insight into molecular mechanisms of cryoinjuries to spermatozoa. Theriogenology, 191():77-95(2022) [pubmed]

submitter keyword: rabbit, MALDI TOF/TOF, 2D-DIGE, spermatozoa

Mariola Słowińska
contact affiliation	Institute of Animal Reproduction and Food Research of Polish Academy of Sciences
contact email	m.slowinska@pan.olsztyn.pl
lab head
Mariola Słowińska
contact affiliation	Institute of Animal Reproduction and Food Research of Polish Academy of Sciences
contact email	m.slowinska@pan.olsztyn.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD034832

PRIDE project URI

• PRIDE
  1. PXD034832
     1. Label: PRIDE project
     2. Name: Proteomic analysis of rabbit fresh and cryopreserved semen (spermatozoa)