PXD034831

PXD034831 is an original dataset announced via ProteomeXchange.

Title	Response of Pseudomonas aeruginosa laboratory strain PAO1 and clinical isolates LESlike1, LESB58, and LES431 to antibiotic treatments
Description	Pseudomonas aeruginosa is a predominant pathogen in chronic lung infections in individuals with cystic fibrosis (CF). Epidemic strains of P. aeruginosa, such as the Liverpool Epidemic Strain (LES), are capable of transferring between CF patients and have been associated with increased hospital visits and antibiotic treatments. We used label-free quantitative proteomics to compare the laboratory strain PAO1, beta-lactam resistant isolate LESB58, and beta-lactam susceptible isolate LESlike1 and their responses to three beta-lactams (aztreonam, carbenicillin, piperacillin), the aminoglycoside tobramycin, and hydrogen peroxide. Across all samples, we identified 3019 proteins with a minimum of two peptides. We found that LESB58 showed a large response to treatment with the beta-lactam carbenicillin, with 644 proteins significantly increased in abundance and 590 proteins significantly decreased in abundance (Students t-test, p≤0.05, FDR=0.05, S0=1). Proteomic characterization of an additional beta-lactam resistant isolate, LES431, exposed to carbenicillin showed that this response was shared by both isolates. Part of the response to carbenicillin in LESB58 included an increase in abundance in proteins involved in cell wall synthesis and division.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:10:26.781.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cezar Khursigara
SpeciesList	scientific name: Pseudomonas aeruginosa LES431; NCBI TaxID: 1408272; scientific name: Pseudomonas aeruginosa LESlike1; NCBI TaxID: 1408274; scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: NCBITaxon:208964; scientific name: Pseudomonas aeruginosa LESB58; NCBI TaxID: 557722;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-22 02:31:46	ID requested
1	2023-08-23 09:27:14	announced
⏵ 2	2023-11-14 07:10:27	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: antibiotics, label-free quantitative proteomics, Liverpool Epidemic Strain,Pseudomonas aeruginosa

Cezar Khursigara
contact affiliation	University of Guelph, Department of Molecular and Cellular Biology
contact email	ckhursig@uoguelph.ca
lab head
Cezar Khursigara
contact affiliation	University of Guelph
contact email	ckhursig@uoguelph.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034831
     1. Label: PRIDE project
     2. Name: Response of Pseudomonas aeruginosa laboratory strain PAO1 and clinical isolates LESlike1, LESB58, and LES431 to antibiotic treatments