PXD034827

PXD034827 is an original dataset announced via ProteomeXchange.

Title	Rat sensory and motor nerve fibroblasts LC-MSMS
Description	In this study, a quantitative proteomic technique based on isobaric tags for relative and absolute quantitation (iTRAQ) was used to compare the proteome of cultured sensory and motor nerve fibroblasts (Fbs). Among a total of 2597 overlapping proteins identified, we obtained 148 differentially expressed proteins, of which 116 proteins were significantly higher expressed in sensory Fbs, and 32 proteins were significantly higher expressed in motor Fbs. Western blot and qPCR analysis were applied to validate differentially expressed proteins. Functional categorization indicated that differentially expressed proteins were linked to a diverse array of biological processes , including regeneration, axon guidance, cytoskeleton organization, cell proliferation, cell migration, cell adhesion, and tissue remodeling, which might play a critical role in the specificity of peripheral nerve regeneration. Furthermore, following co-culture of motor neurons with motor or sensory Fbs , motor Fbs significantly enhanced neurite growth than sensory Fbs. These findings indicated that nerve Fbs expressed the distinct motor and sensory phenotypes involved in different patterns of protein expression, biological processes, and effects on neurons.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:37:09.107.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuhua JI
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-22 02:09:38	ID requested
1	2023-03-10 20:50:04	announced
⏵ 2	2023-11-14 08:37:10	announced	2023-11-14: Updated project metadata.

He Q, Cheng Z, Zhou Q, Tong F, Li Y, Zhou X, Yu M, Ji Y, Ding F, Sensory and motor fibroblasts have different protein expression patterns and exert different growth promoting effects on sensory and motor neurons. Exp Neurol, 361():114314(2023) [pubmed]

submitter keyword: LC-MSMS, Sensory nerve fibroblasts, Motor nerve fibroblasts,Rat

Yuhua Ji
contact affiliation	Department of Immunobiology, College of Life Science and Technology, Jinan University, Guangzhou, China.
contact email	tjiyuhua@126.com
lab head
Yuhua JI
contact affiliation	Department of Immunobiology, College of Life Science and Technology, Jinan University, Guangzhou, China.
contact email	tjiyuhua@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD034827

PRIDE project URI

• PRIDE
  1. PXD034827
     1. Label: PRIDE project
     2. Name: Rat sensory and motor nerve fibroblasts LC-MSMS