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PXD034826

PXD034826 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics analysis for the study of beta cell adaptation to stress in mice deficient for insulin-degrading enzyme (IDE)
DescriptionAppropriate tuning of protein homeostasis through mobilization of the unfolded protein response (UPR) is key to the capacity of pancreatic beta cells to cope with highly variable demand for insulin synthesis. An efficient UPR ensures a sufficient beta cell mass and secretory output but can also affect beta cell resilience to autoimmune aggression. However, the factors regulating protein homeostasis in the face of metabolic and immune challenges are insufficie tly understood. We examined beta cell adaptation to stress in mice deficient for insulin-degrading enzyme (IDE), a ubiquitous protease with high affinity for insulin, a putative ill-defined role in protein homeostasis, and genetic association with type 2 diabetes. IDE deficiency induces a low-level UPR in both standard and autoimmune non-obese diabetic (NOD) mice, associated with rapamycin-sensitive beta cell proliferation, as well as protection from diabetes in NOD mice. Moreover, in NOD islets, IDE deficiency specifically induces strong upregulation of regenerating islet-derived protein 2, a protein attenuating inflammation and protecting from autoimmunity. Our findings establish a role of IDE in islet cell protein homeostasis, corroborate the link between low-level UPR and proliferation, and identify an anti-inflammatory islet cell response uncovered in the absence of IDE of potential interest in autoimmune diabetes.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:45:20.431.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD034826
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterCerina Chhuon
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumenttimsTOF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-22 02:09:19ID requested
12024-06-15 23:22:10announced
22024-10-22 06:45:25announced2024-10-22: Updated project metadata.
Publication List
Zhu S, Waeckel-, É, n, é, e E, Oshima M, Moser A, Bessard MA, Gdoura A, Roger K, Mode N, Lipecka J, Yilmaz A, Bertocci B, Diana J, Saintpierre B, Guerrera IC, Scharfmann R, Francesconi S, Mauvais FX, van Endert P, Islet cell stress induced by insulin-degrading enzyme deficiency promotes regeneration and protection from autoimmune diabetes. iScience, 27(6):109929(2024) [pubmed]
10.1016/j.isci.2024.109929;
10.6019/PXD034826;
Keyword List
submitter keyword: LC-MS/MS, proteomics, protein homeostasis, glucose metabolism, unfolded protein response, autoimmunity, diabetes
Contact List
Ida Chiara Guerrera
contact affiliationLab Head of Necker Proteomics Platform
contact emailchiara.guerrera@inserm.fr
lab head
Cerina Chhuon
contact affiliationProteomics Platform Necker, PPN-3P5, Structure Fédérative de Recherche SFR Necker, Université Paris Descartes, Paris, France
contact emailcerina.chhuon@inserm.fr
dataset submitter
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Dataset FTP location
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PRIDE project URI
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