PXD034821

PXD034821 is an original dataset announced via ProteomeXchange.

Title	Short-term administration of common anesthetics does not dramatically change the endogenous peptide profile in the rat pituitary
Description	To identify and quantify the relative abundances of cell-cell signaling peptides in different physiological states, LC-MS-based peptidomics workflows are commonly utilized on freshly dissected tissue. In such animal experiments, the administration of general anesthetics is an important step for many research projects. However, acute anesthetic administration may rapidly change the measured abundance of transmitter molecules and metabolites which may confound experimental results. The aim of this study was to evaluate the effect of short-term (< 5 min) anesthetic administration on the measured abundance pituitary peptides, as evaluated by a typical peptidomics workflow. To accomplish this goal, we compared peptide abundances in the rat pituitary following administration of 5% isoflurane, 200 mg/kg sodium pentobarbital, or no anesthetic administration using a label-free peptidomics protocol.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:56:43.992.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD034821
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	James Checco
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	2-pyrrolidone-5-carboxylic acid; amidated residue; acetylated residue; monohydroxylated residue
Instrument	Xevo G2-XS QTof

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-22 02:03:35	ID requested
1	2022-09-23 12:00:25	announced
⏵ 2	2023-11-14 08:56:44	announced	2023-11-14: Updated project metadata.

10.6019/PXD034821;

submitter keyword: pituitary, anesthesia, rat,Peptidomics

James Checco
contact affiliation	Department of Chemistry University of Nebraska-Lincoln Lincoln, NE 68588 USA
contact email	checco@unl.edu
lab head
James Checco
contact affiliation	University of Nebraska-Lincoln
contact email	checco@unl.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034821
     1. Label: PRIDE project
     2. Name: Short-term administration of common anesthetics does not dramatically change the endogenous peptide profile in the rat pituitary