PXD034656

PXD034656 is an original dataset announced via ProteomeXchange.

Title	Npl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA
Description	Assessing the function of the RNA-binding activity of RNA-binding proteins (RBPs) is challenging. Here, Keil et al. use a targeted XL-ms approach to identify RNA-binding sites in components of nuclear mRNPs in S. cerevisiae. The analysis of RNA-binding mutants of one selected example, the SR-like protein Npl3, uncovers two novel functions of Npl3, namely in the recruitment of mRNP components to transcribed protein-coding genes and their transfer onto the mRNA. This data set applies label-free protein quantification to assess protein abundancies in NPL3 or CBC2 affinity purification experiments from cellular lysates of NPL3-WT and NPL3-P196D/A197D double-mutant expressing cells.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:43:08.280.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Henning Urlaub
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-19 05:19:18	ID requested
1	2023-03-11 06:29:10	announced
⏵ 2	2023-11-14 08:43:13	announced	2023-11-14: Updated project metadata.

Keil P, Wulf A, Kachariya N, Reuscher S, H, ü, hn K, Silbern I, Altm, ü, ller J, Keller M, Stehle R, Zarnack K, Sattler M, Urlaub H, Str, ä, ß, er K, Npl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA. Nucleic Acids Res, 51(2):831-851(2023) [pubmed]

submitter keyword: affinity purification, RNA-processing, LFQ,NPL3, Spliceosome

Prof. Dr. Henning Urlaub
contact affiliation	Bioanalytical Mass Spectrometry Group, Max-Planck Institute for Multidisciplinary Sciencies, Goettingen, Germany; Institue for Clinical Chemistry, University Medical Center Goettingen, Goettingen, Germany
contact email	henning.urlaub@mpinat.mpg.de
lab head
Henning Urlaub
contact affiliation	Max-Plank Institute for Biophysical Chemistry
contact email	msbio.goettingen@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034656
     1. Label: PRIDE project
     2. Name: Npl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA