PXD034652

PXD034652 is an original dataset announced via ProteomeXchange.

Title	Free fatty acid and α‐lactalbumin‐oleic acid complexes in preterm human milk are cytotoxic to fetal intestinal cells in vitro
Description	Human milk, the best enteral selection for a preterm infant, becomes altered during freezing and soluble free fatty acid is generated over time. Free fatty acids may form complexes, such as the oleic acid-bound protein called HAMLET (human α‐lactalbumin made lethal to tumor cells). We determined the in vitro biological activity of preterm human milk protein‐oleic complexes (HAMLET-like complexes) and tested the hypothesis that laboratory-synthesized HAMLET exhibits cytotoxicity in human immature epithelial intestinal cell culture. Thirty-four milk samples from 15 mothers of hospitalized preterm infants were donated over time. Milk fractions were tested repeatedly for FHs 74 Int and HIEC-6 fetal cell cytotoxicity, using a sensitive viability assay. Protein and fatty acid identities were confirmed by Western blot, high performance liquid chromatography, and mass spectrometry. Cytotoxicity of intestinal cells exposed to milk increased respective to milk storage time (p<0.001) and was associated with free oleic acid (p=0.009). Synthesized HAMLET was cytotoxic in cultures of both lines. Preterm milk samples killed most cells in culture after an average 54 days in frozen storage (95% C.I. 34-72 days). After prolonged storage time, preterm milk and HAMLET showed a degree of cytotoxicity to immature intestinal cells in culture. Protein was reduced 221 in 1 mM dithiothreitol and alkylated in 5.5 mM
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-11_23:49:01.639.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jennifer Bethard
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-19 05:12:34	ID requested
⏵ 1	2022-08-11 23:49:02	announced

Chetta KE, Newton DA, Wagner CL, Baatz JE, . Front Nutr, 9():918872(2022) [pubmed]

submitter keyword: human milk, free fatty acid, HAMLET (human alpha-lactalbumin made lethal to tumor cells), cytotoxic, prematurity, Growth, Oleic Acid, ARA - 20:4n-6, Lactoferrin, Alpha-lactalbumin, Fetal intestinal epithelium, Necrotizing entercolitis

Katherine Chetta
contact affiliation	Pediatrics and Neonatology Medical University of South Carolina
contact email	chetta@musc.edu
lab head
Jennifer Bethard
contact affiliation	Medical University of SC
contact email	bethard@musc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034652
     1. Label: PRIDE project
     2. Name: Free fatty acid and α‐lactalbumin‐oleic acid complexes in preterm human milk are cytotoxic to fetal intestinal cells in vitro