PXD034652 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Free fatty acid and α‐lactalbumin‐oleic acid complexes in preterm human milk are cytotoxic to fetal intestinal cells in vitro |
Description | Human milk, the best enteral selection for a preterm infant, becomes altered during freezing and soluble free fatty acid is generated over time. Free fatty acids may form complexes, such as the oleic acid-bound protein called HAMLET (human α‐lactalbumin made lethal to tumor cells). We determined the in vitro biological activity of preterm human milk protein‐oleic complexes (HAMLET-like complexes) and tested the hypothesis that laboratory-synthesized HAMLET exhibits cytotoxicity in human immature epithelial intestinal cell culture. Thirty-four milk samples from 15 mothers of hospitalized preterm infants were donated over time. Milk fractions were tested repeatedly for FHs 74 Int and HIEC-6 fetal cell cytotoxicity, using a sensitive viability assay. Protein and fatty acid identities were confirmed by Western blot, high performance liquid chromatography, and mass spectrometry. Cytotoxicity of intestinal cells exposed to milk increased respective to milk storage time (p<0.001) and was associated with free oleic acid (p=0.009). Synthesized HAMLET was cytotoxic in cultures of both lines. Preterm milk samples killed most cells in culture after an average 54 days in frozen storage (95% C.I. 34-72 days). After prolonged storage time, preterm milk and HAMLET showed a degree of cytotoxicity to immature intestinal cells in culture. Protein was reduced 221 in 1 mM dithiothreitol and alkylated in 5.5 mM |
HostingRepository | PRIDE |
AnnounceDate | 2022-08-12 |
AnnouncementXML | Submission_2022-08-11_23:49:01.639.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jennifer Bethard |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-06-19 05:12:34 | ID requested | |
⏵ 1 | 2022-08-11 23:49:02 | announced | |
Publication List
Chetta KE, Newton DA, Wagner CL, Baatz JE, . Front Nutr, 9():918872(2022) [pubmed] |
Keyword List
submitter keyword: human milk, free fatty acid, HAMLET (human alpha-lactalbumin made lethal to tumor cells), cytotoxic, prematurity, Growth, Oleic Acid, ARA - 20:4n-6, Lactoferrin, Alpha-lactalbumin, Fetal intestinal epithelium, Necrotizing entercolitis |
Contact List
Katherine Chetta |
contact affiliation | Pediatrics and Neonatology Medical University of South Carolina |
contact email | chetta@musc.edu |
lab head | |
Jennifer Bethard |
contact affiliation | Medical University of SC |
contact email | bethard@musc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034652
- Label: PRIDE project
- Name: Free fatty acid and α‐lactalbumin‐oleic acid complexes in preterm human milk are cytotoxic to fetal intestinal cells in vitro